Near-Infrared II Gold Nanocluster Assemblies with Improved Luminescence and Biofate for In Vivo Ratiometric Imaging of H2S
Near-Infrared II Gold Nanocluster Assemblies with Improved Luminescence and Biofate for In Vivo Ratiometric Imaging of H2S
复制标题
具有改进的发光和生物命运的近红外 II 金纳米团簇组件，用于 H(2)S 体内比例成像。
DOI：
10.1021/acs.analchem.1c05154
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发表时间：
2022-01-27
影响因子：
7.4
通讯作者：
Yang, Huanghao
中科院分区：
文献类型：
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作者：
Li, Shihua;Ma, Qiuping;Yang, Huanghao
超小金纳米团簇（aunc）由于其出色的光致发光（PL）和肾脏清除能力而成为生物成像领域有前途的发光纳米探针。然而，如何设计出适合于所需组织的体内敏感分子成像技术仍然是一个巨大的挑战。在此，我们开发了一种策略，通过配体锚定来定制近红外II （NIR-II）发射AuNCs的PL和生物酸盐，以改善生物成像。通过优化AuNCs中的配体类型，并以Er3+掺杂镧系元素（Ln）纳米颗粒为模型，合理构建了核心-卫星Ln@AuNCs组件，使AuNCs在1100 nm处的PL增强2.5倍，并延长了血液循环。值得注意的是，Ln@AuNCs具有双重强度的NIR-II PL（来自AuNCs和Er3+）可以有效地积聚在肝脏中，用于H2S的比例NIR-II成像，H2S介导的AuNCs选择性PL猝灭促进了这一点。然后，我们展示了两种H2S前药的肝脏递送效果和动态的实时成像评估。这显示了一种可视化肝脏H2S输送及其体内前药筛选的范例。请注意，Ln@AuNCs可通过肝胆排泄途径被人体清除，从而降低潜在的长期毒性。这些发现可能会推动AuNC纳米探针的工程化，以推进体内生物成像分析。
Ultrasmall gold nanoclusters (AuNCs) are emerging as promising luminescent nanoprobes for bioimaging due to their fantastic photoluminescence (PL) and renal-clearable ability. However, it remains a great challenge to design them for in vivo sensitive molecular imaging in desired tissues. Herein, we have developed a strategy to tailor the PL and biofate of near-infrared II (NIR-II)emitting AuNCs via ligand anchoring for improved bioimaging. By optimizing the ligand types in AuNCs and using Er3+-doped lanthanide (Ln) nanoparticles as models, core-satellite Ln@AuNCs assemblies were rationally constructed, which enabled 2.5-fold PL enhancement of AuNCs at 1100 nm and prolonged blood circulation compared to AuNCs. Significantly, Ln@AuNCs with dual intense NIR-II PL (from AuNCs and Er3+) can effectively accumulate in the liver for ratiometric NIR-II imaging of H2S, facilitated by H2S-mediated selective PL quenching of AuNCs. We have then demonstrated the real-time imaging evaluation of liver delivery efficacy and dynamics of two H2S prodrugs. This shows a paradigm to visualize liver H2S delivery and its prodrug screening in vivo. Note that Ln@AuNCs are body-clearable via the hepatobiliary excretion pathway, thus reducing potential long-term toxicity. Such findings may propel the engineering of AuNC nanoprobes for advancing in vivo bioimaging analysis.