EVALUATION OF MOUSE MONOCLONAL-ANTIBODIES RAISED AGAINST AN ISOLATE OF THE VARIANT AVIAN PARAMYXOVIRUS TYPE-1 RESPONSIBLE FOR THE CURRENT PANZOOTIC IN PIGEONS
EVALUATION OF MOUSE MONOCLONAL-ANTIBODIES RAISED AGAINST AN ISOLATE OF THE VARIANT AVIAN PARAMYXOVIRUS TYPE-1 RESPONSIBLE FOR THE CURRENT PANZOOTIC IN PIGEONS
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DOI：
10.1007/bf01313807
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发表时间：
1989-01-01
影响因子：
2.7
通讯作者：
MANVELL, RJ
中科院分区：
文献类型：
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作者：
COLLINS, MS;ALEXANDER, DJ;MANVELL, RJ
针对“鸽变体”禽副粘病毒1型分离株pigeon/England/617/83产生的9种单克隆抗体测试了它们与“经典”和其它“鸽变体”分离株反应的能力。其中两种单克隆抗体似乎对617/83具有特异性，不与其他病毒反应。其余7个单克隆抗体绑定到感染所有其他“鸽子”在间接免疫过氧化物酶（IIP）测试中分离的细胞，但其他PMV-1病毒的四个不同的组的结合模式的基础上形成。其中一种单克隆抗体161/617引起了所有分离的试验“鸽子”的血凝抑制（HI），但没有引起其它PMV-1病毒的血凝抑制，这些结果反映了用该单克隆抗体的IIP结果。在HI试验中，161/617还显示出抑制禽副粘病毒3型血清组的病毒。在IIP试验中，这种反应和与感染细胞结合的能力似乎仅限于从外来鸟类中分离的PMV-3病毒，而与火鸡中表面上相同血清型的病毒没有发生。
Nine monoclonal antibodies raised against "pigeon variant" avian paramyxovirus type 1 isolate pigeon/England/617/83 were tested for their ability to react with "classical" and other "pigeon variant" isolates. Two of the monoclonal antibodies appeared to be specific for 617/83 reacting with no other virus. The remaining seven monoclonal antibodies bound to cells infected with all other "pigeon" isolated in indirect immunoperoxidase (IIP) tests but four distinct groups of other PMV-1 viruses were formed on the basis of the binding patterns. one of the monoclonal antibodies 161/617 caused haemagglutination inhibition (HI) of all the "pigeon" isolated tested but none of the other PMV-1 viruses and these results reflected the IIP results with this monoclonal antibody. 161/617 was also shown to inhibit viruses of the avian paramyxovirus type 3 serogroup in HI tests. This reaction and the ability to bind to infected cells in IIP tests appeared to be restricted to PMV-3 viruses isolated from exotic birds and did not occur with virus of ostensively the same serotype from turkey.