RT-PCR and sequence analysis of the full-length fusion protein of Canine Distemper Virus from domestic dogs
RT-PCR and sequence analysis of the full-length fusion protein of Canine Distemper Virus from domestic dogs
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DOI：
10.1016/j.jviromet.2015.11.011
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发表时间：
2016-02-01
影响因子：
3.1
通讯作者：
La Torre, Jose
中科院分区：
文献类型：
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作者：
Romanutti, Carina;Gallo Calderon, Marina;La Torre, Jose
在2007-2014年期间，提交给我们实验室用于诊断目的的236份家犬样本中有84份（35.6%）对犬瘟热病毒（CDV）呈阳性，通过RT-PCR扩增核蛋白基因片段进行分析。其中59只（70.2%）来自接种过犬瘟热疫苗的犬。对15个分离株的编码融合（F）蛋白的全长基因进行测序，并与其他CDV（包括野生型和疫苗株）的编码融合（F）蛋白的全长基因进行比较。使用F基因全长序列的系统发育分析将所有阿根廷CDV株在SA 2 Glade中分组。与Onderstepoort疫苗株的序列同一性为89.0- 90.6%，并且在对应于F蛋白信号肽Fsp的135个氨基酸中发现最高的差异（64.4-66.7%同一性）。相反，该区域在本地菌株中高度保守（94.1-100%同一性）。与疫苗株相比，在CDV阿根廷株的F基因中鉴定出一个额外的推定N-糖基化位点。本报告是第一个分析全长F蛋白序列的CDV株在阿根廷流通，并有助于CDV的分子流行病学的知识，这可能有助于了解未来的疾病暴发。(C)2015 Elsevier B. V.版权所有。
During 2007-2014, 84 out of 236 (35.6%) samples from domestic dogs submitted to our laboratory for diagnostic purposes were positive for Canine Distemper Virus (CDV), as analyzed by RT-PCR amplification of a fragment of the nucleoprotein gene. Fifty-nine of them (70.2%) were from dogs that had been vaccinated against CDV. The full-length gene encoding the Fusion (F) protein of fifteen isolates was sequenced and compared with that of those of other CDVs, including wild-type and vaccine strains. Phylogenetic analysis using the F gene full-length sequences grouped all the Argentinean CDV strains in the SA2 Glade. Sequence identity with the Onderstepoort vaccine strain was 89.0-90.6%, and the highest divergence was found in the 135 amino acids corresponding to the F protein signal-peptide, Fsp (64.4-66.7% identity). In contrast, this region was highly conserved among the local strains (94.1-100% identity). One extra putative N-glycosylation site was identified in the F gene of CDV Argentinean strains with respect to the vaccine strain. The present report is the first to analyze full-length F protein sequences of CDV strains circulating in Argentina, and contributes to the knowledge of molecular epidemiology of CDV, which may help in understanding future disease outbreaks. (C) 2015 Elsevier B.V. All rights reserved.