Fluorescent proteins expressed in mouse transgenic lines mark subsets of glia, neurons, macrophages, and dendritic cells for vital examination
Fluorescent proteins expressed in mouse transgenic lines mark subsets of glia, neurons, macrophages, and dendritic cells for vital examination
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DOI：
10.1523/jneurosci.3934-04.2004
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发表时间：
2004-12-08
影响因子：
5.3
通讯作者：
Thompson, WJ
中科院分区：
文献类型：
--
作者：
Zuo, Y;Lubischer, JL;Thompson, WJ
为了能够在神经肌肉接头处对神经胶质细胞进行重要观察，产生了在人S100 B基因的转录调控序列的控制下表达绿色荧光蛋白家族的蛋白质的转基因小鼠。终端许旺细胞反复成像在活体动物的转基因线路之一，以显示，除了延伸和收缩的短过程，神经胶质覆盖的成年神经肌肉突触是稳定的。在其他细胞系中，标记了许旺细胞的亚群。标记的分布表明，单个突触处的施万细胞是克隆相关的，这一发现对这些细胞在出生后发育过程中如何分类具有影响。其他标记模式，一些存在于独特的线，包括星形胶质细胞，小胶质细胞，小脑Bergmann胶质细胞，脊髓运动神经元，巨噬细胞和树突状细胞的子集。我们表明，标记的巨噬细胞系可用于跟踪这些细胞在损伤部位的积累。
To enable vital observation of glia at the neuromuscular junction, transgenic mice were generated that express proteins of the green fluorescent protein family under control of transcriptional regulatory sequences of the human S100B gene. Terminal Schwann cells were imaged repetitively in living animals of one of the transgenic lines to show that, except for extension and retraction of short processes, the glial coverings of the adult neuromuscular synapse are stable. In other lines, subsets of Schwann cells were labeled. The distribution of label suggests that Schwann cells at individual synapses are clonally related, a finding with implications for how these cells might be sorted during postnatal development. Other labeling patterns, some present in unique lines, included astrocytes, microglia, and subsets of cerebellar Bergmann glia, spinal motor neurons, macrophages, and dendritic cells. We show that lines with labeled macrophages can be used to follow the accumulation of these cells at sites of injury.