Characterization of chromatophorotropic neuropeptides from the kuruma prawn Penaeus japonicus.
Characterization of chromatophorotropic neuropeptides from the kuruma prawn Penaeus japonicus.
复制标题
来自日本对虾的趋色神经肽的表征。
DOI：
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发表时间：
1999
影响因子：
2.7
通讯作者：
Hiromichi Nagasawa
中科院分区：
文献类型：
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作者：
Wei;Katsumi Aida;Hiromichi Nagasawa
用两步反相高效液相色谱法从日本对虾鼻窦腺体水提物中分离纯化了3种致色神经肽激素，并测定了它们的氨基酸序列。其中一种具有浓缩色素活性，氨基酸序列与已知的红色素浓缩激素(RPCH)相同，故命名为Pej-RPCH。另两个具有色素分散激素(PDH)活性的多肽分别命名为Pej-PDH-I和-II。Pej-PDH-I和-II的序列分别为NSELINSLLGIPKVMTDAAME和NSELINSLLGLPKFMIDA酰胺。两种PDH在11、14和16位的三个氨基酸残基不同。PEJ-PDH-II对红团、黄团和黑素团的效力分别是PeJ-PDH-I的5倍、7倍和10倍。这两种PDH效价差异的主要原因是第16位残基的差异。此外，还发现它们是由单个个体产生的。四种类型的发色团对Pej-RPCH和两种PDHs的敏感性顺序为：红发光团=黄发光团&黑素发光团&白发光团。
Three chromatophorotropic neuropeptide hormones were purified from an aqueous extract of the sinus glands of the kuruma prawn Penaeus japonicus by two steps of reverse-phase HPLC and their amino acid sequences determined. One of them was found to show pigment concentrating activity and to have an amino acid sequence identical with that of the known red pigment concentrating hormone (RPCH), and therefore it was named Pej-RPCH. The other two peptides showed pigment dispersing hormone (PDH) activity and were named Pej-PDH-I and -II. They both consisted of 18 amino acid residues with a free amino-terminus and an amidated carboxyl-terminus, the sequences of Pej-PDH-I and -II being NSELINSLLGIPKVMTDAamide and NSELINSLLGLPKFMIDAamide, respectively. Three amino acid residues at positions 11, 14, and 16 differed between the two PDHs. Pej-PDH-II was about 5-, 7-, and 10-fold more potent than Pej-PDH-I for erythrophores, xanthophores, and melanophores, respectively. The major reason for the difference in potency between the two PDHs was attributed to differences in residues at position 16. In addition, they were found to be produced by a single individual. The order of sensitivity of the four types of chromatophores to Pej-RPCH and both PDHs was found to be erythrophores = xanthophores > melanophores > leukophores.