In vivo induction of apoptosis and immune responses in mice by administration of lipopolysaccharide from Porphyromonas gingivalis
In vivo induction of apoptosis and immune responses in mice by administration of lipopolysaccharide from Porphyromonas gingivalis
复制标题
DOI：
10.1128/iai.64.4.1461-1466.1996
复制
发表时间：
1996-04-01
影响因子：
3.1
通讯作者：
Hayashi, M
中科院分区：
文献类型：
--
作者：
Isogai, E;Isogai, H;Hayashi, M
在小鼠体内给予牙龈卟啉单胞菌脂多糖(PG-LPS)诱导细胞凋亡，然后进行特异性免疫反应。细胞凋亡与B细胞免疫球蛋白和Ia、T细胞CD5和多种标志物的表达有关。肿瘤坏死因子-α(TNF-α)在细胞凋亡前1d显著高于对照组，重组小鼠肿瘤坏死因子-α可诱导细胞凋亡，因此肿瘤坏死因子-α可介导细胞凋亡。Pg-内毒素诱导小鼠胸腺T细胞凋亡的基本方式与大肠杆菌内毒素相似，但胸腺T细胞对Pg-内毒素诱导的胸腺T细胞的免疫应答是独特的。提示PG-内毒素可诱导细胞凋亡，从而消除早期非特异性激活的淋巴细胞。
In vivo administration of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) to mice induced apoptosis before a specific immune response. Apoptosis was associated with the expression of immunoglobulin and Ia on B cells and of CD5 and several markers on T cells. Apoptosis peaked in the spleen and lymph nodes on day 2, and the second peak occurred in the thymus on day 9. Tumor necrosis factor alpha (TNF-alpha) could mediate apoptosis, because the serum TNF-alpha levels were significantly higher than those of controls at 1 day before apoptosis and recombinant murine TNF-alpha induced apoptosis. The apoptosis induced by Pg-LPS was similar to that induced by Escherichia coli LPS in its basic manner, but it was unique in the response of thymus T cells. It was suggested that Pg-LPS could induce apoptosis for the elimination of early nonspecific activated lymphocytes.