ISOLATION AND INACTIVATION OF THE NUCLEAR GENE ENCODING THE ROTENONE-INSENSITIVE INTERNAL NADH - UBIQUINONE OXIDOREDUCTASE OF MITOCHONDRIA FROM SACCHAROMYCES-CEREVISIAE
ISOLATION AND INACTIVATION OF THE NUCLEAR GENE ENCODING THE ROTENONE-INSENSITIVE INTERNAL NADH - UBIQUINONE OXIDOREDUCTASE OF MITOCHONDRIA FROM SACCHAROMYCES-CEREVISIAE
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DOI：
10.1111/j.1432-1033.1991.tb15775.x
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发表时间：
1991-02-14
期刊：
影响因子：
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通讯作者：
GRIVELL, LA
中科院分区：
文献类型：
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作者：
MARRES, CAM;DEVRIES, S;GRIVELL, LA
我们最近描述了从酿酒酵母中分离线粒体鱼藤酮不敏感的NADH：泛醌氧化还原酶[de弗里斯，S. & Grivell，L. A.（1988）Eur. 176，377-384]。 我们现在报告的核基因编码这种单亚基酶的分离。 已经通过一步基因破坏的方式构建了突变体。 用从突变细胞中分离的线粒体进行的摄氧实验表明，这种NADH脱氢酶催化线粒体内产生的NADH的氧化。 这种NADH脱氢酶的失活不影响葡萄糖和乙醇上的生长，但乳酸盐、丙酮酸盐和乙酸盐上的生长受损或不存在。 这种表型是讨论在酵母中不同的代谢途径之间的相互作用。
We have recntly described the isolation of a mitochondrial rotenone-insensitive NADH:ubiquinone oxidoreductase from Saccharomyces cerevisiae [de Vries, S. & Grivell, L. A. (1988) Eur. J. Biochem. 176, 377-384]. We now report the isolation of the nuclear gene encoding this single-subunit enzyme. Null mutants have been constructed by means of one-step gene disruption. Oxygen-uptake experiments, performed with mitochondria isolated from the mutant cells, showed that this NADH dehydrogenase catalyzes the oxidation of NADH generated inside the mitochondrion. Inactivation of this NADH dehydrogenase does not affect growth on glucose and ethanol, but growth on lactate, pyruvate and acetate is impaired or absent. This phenotype is discussed in terms of the interplay between different metabolic pathways in yeast.