ENDOTHELIAL-CELL GENE-EXPRESSION OF A NEUTROPHIL CHEMOTACTIC FACTOR BY TNF-ALPHA, LPS, AND IL-1-BETA
ENDOTHELIAL-CELL GENE-EXPRESSION OF A NEUTROPHIL CHEMOTACTIC FACTOR BY TNF-ALPHA, LPS, AND IL-1-BETA
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DOI：
10.1126/science.2648570
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发表时间：
1989-03-17
期刊：
影响因子：
56.9
通讯作者：
MARKS, RM
中科院分区：
文献类型：
--
作者：
STRIETER, RM;KUNKEL, SL;MARKS, RM
人内皮细胞在肿瘤坏死因子刺激下产生中性粒细胞趋化因子(NCF)。(肿瘤坏死因子-α)、白介素1-β。(IL-1β)，或脂多糖(LPS)。内皮细胞来源的NCF信使RNA的表达和生物活性都具有时间和浓度依赖性。NCFmRNA的最大表达分别出现在10和2纳克/毫升的肿瘤坏死因子和白介素1β；在刺激后1小时首次观察到mRNA的表达，并维持至少24小时。原位杂交分析显示，NCFmRNA在处理后24小时达高峰，而未刺激细胞则为阴性。这些研究表明，内皮细胞可能通过合成一种趋化因子来响应特定的单因子和内毒素，从而参与中性粒细胞介导的炎症。
Human endothelial cells produced a neutrophil chemotactic factor (NCF) upon stimulation with tumor necrosis factor.sbd..alpha. (TNF-.alpha.), interleukin-1.beta. (IL-1.beta.), or lipopolysaccharide (LPS). The expression of endothelial cell-derived NCF messenger RNA and biological activity was both time- and concentration-dependent. Maximal NCF mRNA expression occurred at 10 and at 2 nanograms per milliliter for TNF and IL-1.beta., respectively; mRNA expression was first observed 1 hour after stimulation and was maintained for at least 24 hours. In situ hybridization analysis showed that NCF mRNA peaked in treated cells by 24 hours, whereas unstimulated cells were negative. These studies demonstrated that endothelial cells may participate in neutrophil-mediated inflammation by synthesizing a chemotactic factor in response to specific monokines and LPS.