Effects of the blended fibroin/aloe gel film on wound healing in streptozotocin-induced diabetic rats
Effects of the blended fibroin/aloe gel film on wound healing in streptozotocin-induced diabetic rats
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DOI：
10.1088/1748-6041/7/3/035008
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发表时间：
2012-06-01
影响因子：
4
通讯作者：
Viyoch, Jarupa
中科院分区：
文献类型：
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作者：
Inpanya, Paichit;Faikrua, Atchariya;Viyoch, Jarupa
延迟愈合仍然是一个主要的临床问题，在这里，我们试图开发一种改进的敷料膜，包括1.95% w/v的丝素和0.05% w/v的芦荟凝胶提取物。干膜拉伸强度为21.1 +/- 0.5 MPa，延伸率为1.1 +/- 0.2%时断裂；湿膜对应值分别为18.3 +/- 1.3 MPa和1.9 +/- 0.1%。水浸后膜保持原状，干膜溶胀率为0.8 +/- 0.1，吸水率为43.7 +/- 2.6%。在磷酸盐缓冲盐水（1 M, pH 7.4, 37℃）中培养28天后，膜的重量减少了6.7 +/- 1.1%，断裂点（干态）的拉伸强度和伸长率分别为15.4 +/- 0.6 MPa和1.5 +/- 0.2%。与不含芦荟的纤维蛋白膜（仅含2.0%的纤维蛋白提取物）相比，混合膜增强了皮肤成纤维细胞的附着和增殖。在维持α -平滑肌肌动蛋白的情况下，混合膜上培养成纤维细胞的bFGF免疫荧光明显大于组织培养板或无芦荟纤维蛋白膜上培养的成纤维细胞。在链脲佐菌素诱导的糖尿病大鼠中，与未处理的糖尿病创面相比，混合膜敷料后第7天创面较小（p < 0.05）。修复后的糖尿病创面组织学显示成纤维细胞分布和胶原纤维组织与正常大鼠创面相似，这与羟脯氨酸含量增加相吻合。因此，这种纤维蛋白/芦荟凝胶混合膜的加速伤口愈合可能应用于糖尿病性皮肤溃疡的治疗。
Delayed healing remains a major clinical problem and here we have sought to develop an improved dressing film comprising 1.95% w/v fibroin and 0.05% w/v aloe gel extract. The tensile strength of dry film was 21.1 +/- 0.5 MPa and broke at 1.1 +/- 0.2% elongation; corresponding values for wet film were 18.3 +/- 1.3 MPa and 1.9 +/- 0.1%. The film maintained its shape upon water immersion and the swelling ratio of the dry film was 0.8 +/- 0.1 while the water uptake was 43.7 +/- 2.6%. After 28 days of incubation in phosphate buffered saline (1 M, pH 7.4, 37 degrees C), the weight of film was reduced by 6.7 +/- 1.1% and the tensile strength and elongation at breaking point (dry state) were 15.4 +/- 0.6 MPa and 1.5 +/- 0.2%, respectively. Compared to aloe-free fibroin film (2.0% fibroin extract only), the blended film enhanced the attachment and proliferation of skin fibroblasts. The bFGF immunofluorescence of fibroblasts cultured on the blended film appeared greater than those cultured on tissue culture plate or on aloe-free fibroin film while alpha-smooth muscle actin was maintained. In streptozotocin-induced diabetic rats, the wounds dressed with the blended film were smaller (p < 0.05) by day 7 after wounding, compared to untreated diabetic wounds. Histology of repaired diabetic wounds showed the fibroblast distribution and collagen fiber organization to be similar to wounds in normal rats, and this was matched by enhanced hydroxyproline content. Thus, such accelerated wound healing by the blended fibroin/aloe gel films may find application in treatment of diabetic non-healing skin ulcers.