Mesenchymal stem cells attenuate ischemia-reperfusion injury after prolonged cold ischemia in a mouse model of lung transplantation: a preliminary study.
Mesenchymal stem cells attenuate ischemia-reperfusion injury after prolonged cold ischemia in a mouse model of lung transplantation: a preliminary study.
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间充质干细胞减轻肺移植小鼠模型长期冷缺血后的缺血再灌注损伤：一项初步研究。
DOI：
10.1007/s00595-016-1391-8
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发表时间：
2017
期刊：
影响因子：
2.5
通讯作者：
Okada Y.
中科院分区：
文献类型：
--
作者：
Watanabe T;Hoshikawa Y;Ishibashi N;Suzuki H;Notsuda H;Watanabe Y;Noda M;Kanehira M;Ohkouchi S;Kondo T;Okada Y.
目的间充质干细胞（MSCs）具有抗炎和免疫作用。方法C57 BL/6 J小鼠肺移植模型，采用低钾右旋糖酐葡萄糖液灌流，4 °C保存18 h。将人骨髓间充质干细胞缓慢注入肺移植物的左肺动脉，然后进行原位左肺移植。同种肺移植物再灌注6 h，收集左肺移植物支气管肺泡灌洗液（BALF）。我们测量的蛋白质浓度，细胞计数，并在BALF中的促炎细胞因子浓度。ResultsThe蛋白质浓度和细胞计数在BALF中的MSC管理的移植物显着低于PBS管理的控制。BALF中促炎细胞因子IL-1β、IL-17 A和TNF-α的浓度在MSC移植的移植物中倾向于低于对照组，但差异不显著。结论移植前通过肺移植物的肺动脉给予MSC减轻了小鼠肺移植模型中长时间冷缺血后的缺血-再灌注损伤。
PurposeMesenchymal stem cells (MSCs) suppress inflammation and immune responses. We conducted this study to find out if MSCs attenuate ischemia–reperfusion injury in a mouse model of lung transplantation.MethodsC57BL/6J mouse lungs perfused with low-potassium dextran glucose solution were preserved at 4 °C for 18 h. Human MSCs were slowly injected into the left pulmonary artery of the lung grafts, and orthotopic left lung transplantation was then performed. The lung isografts were reperfused for 6 h, and bronchoalveolar lavage fluid (BALF) from the left lung graft was collected. We measured the protein concentration, cell count, and proinflammatory cytokine concentrations in the BALF.ResultsThe protein concentration and cell count in the BALF were significantly lower in the MSC-administered grafts than in the PBS-administered controls. Concentrations of proinflammatory cytokines, including IL-1β, IL-17A, and TNF-α, in BALF tended to be lower in the MSC-administered grafts than in the controls, but the difference was not significant.ConclusionThe pre-transplant administration of MSCs via the pulmonary artery of the lung graft attenuated ischemia–reperfusion injury after prolonged cold ischemia in this mouse model of lung transplantation.