Comparison of iron-reduced and iron-supplemented semisynthetic diets in T cell transfer colitis
Comparison of iron-reduced and iron-supplemented semisynthetic diets in T cell transfer colitis
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DOI：
10.1371/journal.pone.0218332
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发表时间：
2019-07-05
期刊：
影响因子：
3.7
通讯作者：
Krug, Anne B.
中科院分区：
文献类型：
--
作者：
Markota, Anamarija;Metzger, Rebecca;Krug, Anne B.
对炎症性肠病患者的临床观察和对啮齿动物的实验研究表明，来自富铁食物或口服补铁的肠腔中的铁可能会加剧炎症，而饮食中的铁消耗可能会起到保护作用。为验证膳食铁降低对结肠炎的保护作用这一假设，研究了膳食铁降低到10 mg/kg以下对成年C57BL/6小鼠CD4+CD62L+T细胞转移性结肠炎病程的影响。用铁含量为10毫克/千克的半合成饲料或添加180毫克/千克的硫酸亚铁或氯化血红素的半合成饲料处理的小鼠，体重减轻以及临床和组织学炎症体征相似。饲料中铁含量不影响致炎大鼠结肠中Ly6C(高)单核细胞的积聚和激活、树突状细胞亚群组成的变化以及致炎Th1/Th17细胞的诱导。因此，在T细胞转移诱导的结肠炎中，饮食中的铁减少并不能保护成年小鼠免受严重的肠道炎症。
Clinical observations in inflammatory bowel disease patients and experimental studies in rodents suggest that iron in the intestinal lumen derived from iron-rich food or oral iron supplementation could exacerbate inflammation and that iron depletion from the diet could be protective. To test the hypothesis that dietary iron reduction is protective against colitis development, the impact of iron reduction in the diet below 10 mg/kg on the course of CD4+ CD62L+ T cell transfer colitis was investigated in adult C57BL/6 mice. Weight loss as well as clinical and histological signs of inflammation were comparable between mice pretreated with semisynthetic diets with either < 10mg/kg iron content or supplemented with 180 mg/kg iron in the form of ferrous sulfate or hemin. Accumulation and activation of Ly6C(high) monocytes, changes in dendritic cell subset composition and induction of proinflammatory Th1/Th17 cells in the inflamed colon were not affected by the iron content of the diets. Thus, dietary iron reduction did not protect adult mice against severe intestinal inflammation in T cell transfer induced colitis.