Single-molecule FRET study of SNARE-mediated membrane fusion.
Single-molecule FRET study of SNARE-mediated membrane fusion.
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DOI：
10.1042/bsr20110011
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发表时间：
2011-12
影响因子：
4
通讯作者：
Bae WR
中科院分区：
文献类型：
--
作者：
Diao J;Ishitsuka Y;Bae WR
膜融合是最重要的细胞过程之一，两个最初不同的脂质双分子层融合它们的疏水核心，形成一个相互连接的结构。被称为SNARE的蛋白质在融合过程中起着核心作用，该过程还受到一些辅助蛋白质的调节。为了研究snare介导的膜融合，涉及集合荧光共振能量转移FRET的体外蛋白质重构实验已经使用了十多年。在这篇小型综述中，我们描述了几种基于单分子FRET的方法，这些方法已应用于该领域，以克服在蛋白质和融合动力学方面的大量分析的不足。
Membrane fusion is one of the most important cellular process by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. Proteins, called SNARE, play a central role in the fusion process which is also regulated by several accessory proteins. In order to study the SNARE-mediated membrane fusion, the in vitro protein reconstitution assay involving ensemble fluorescence resonance energy transfer FRET has been used over a decade. In this mini-review, we describe several single-molecule-based FRET approaches that have been applied to this field to overcome the shortage of the bulk assay in terms of protein and fusion dynamics.