Comparative Transcriptomes Analysis of Red- and White-Fleshed Apples in an F1 Population of Malus sieversii f. niedzwetzkyana Crossed with M. domestica 'Fuji'.
Comparative Transcriptomes Analysis of Red- and White-Fleshed Apples in an F1 Population of Malus sieversii f. niedzwetzkyana Crossed with M. domestica 'Fuji'.
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DOI：
10.1371/journal.pone.0133468
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Wang N;Zheng Y;Duan N;Zhang Z;Ji X;Jiang S;Sun S;Yang L;Bai Y;Fei Z;Chen X
使用新一代高通量 RNA 测序 (RNA-Seq) 技术生成并比较了 Malus sieversii f.Niedzwetzkyana 和 M.domestica ‘Fuji’ F1 分离群体中红肉和白肉苹果的转录组图谱。共获得114个差异表达基因（DEG），其中88个在红肉苹果中上调，26个下调。 88个上调基因富含与类黄酮生物合成过程和应激反应相关的基因。进一步分析确定了 22 个与类黄酮生物合成过程相关的基因和 68 个可能与应激反应相关的基因。此外，通过实时定量PCR验证了20个上调候选基因（10个与类黄酮生物合成相关、2个编码MYB转录因子和8个与应激反应相关）和10个下调基因的表达。在探索了可能的调控网络后，我们推测类黄酮代谢可能参与红肉苹果的应激反应。我们的研究结果为进一步丰富与果树类黄酮合成和胁迫反应相关的基因资源，以及培育具有高类黄酮含量和/或增强抗逆性的优良苹果提供了理论依据。
Transcriptome profiles of the red- and white-fleshed apples in an F1 segregating population of Malus sieversii f.Niedzwetzkyana and M.domestica ‘Fuji’ were generated using the next-generation high-throughput RNA sequencing (RNA-Seq) technology and compared. A total of 114 differentially expressed genes (DEGs) were obtained, of which 88 were up-regulated and 26 were down-regulated in red-fleshed apples. The 88 up-regulated genes were enriched with those related to flavonoid biosynthetic process and stress responses. Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses. Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR. After exploring the possible regulatory network, we speculated that flavonoid metabolism might be involved in stress responses in red-fleshed apple. Our findings provide a theoretical basis for further enriching gene resources associated with flavonoid synthesis and stress responses of fruit trees and for breeding elite apples with high flavonoid content and/or increased stress tolerances.