The stimulatory effect of different CpG oligonucleotides on the maturation of chicken bone marrow-derived dendritic cells
The stimulatory effect of different CpG oligonucleotides on the maturation of chicken bone marrow-derived dendritic cells
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不同CpG寡核苷酸对鸡骨髓源性树突状细胞成熟的刺激作用。
DOI：
10.3382/ps.2013-03431
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发表时间：
2014-01-01
期刊：
影响因子：
4.4
通讯作者：
Yang, Qian
中科院分区：
文献类型：
--
作者：
Fu, Jia;Liang, Jinfeng;Yang, Qian
CpG寡核苷酸（CpG-ODN）可对树突状细胞（DC）等多种免疫细胞发挥免疫刺激作用。CpG-ODN的免疫刺激活性与其核苷酸序列和结构特征密切相关。本研究旨在探讨不同CpG-ODN对鸡骨髓来源DC（BM-DC）体外成熟的促进作用。首先，设计了4个CpG-ODN。从鸡胫骨和股骨中提取骨髓细胞，在含有重组鸡粒细胞-巨噬细胞集落刺激因子（GM-CSF）和IL-4的RPMI 1640培养基中培养。培养6 d后，分别用不同CpG-ODN或脂多糖刺激细胞24 h。最后，通过形态学、表型和功能测定研究了不同CpG-ODN对鸡BM-DC成熟的影响。结果显示，体外培养的BM-DC具有典型的DC形态，CpG-ODN能有效地刺激BM-DC向成熟的形态特征分化，并上调CD 40和CD 86分子的表达。经CpG-ODN刺激后，BM-DC可明显诱导T细胞增殖反应（P < 0.01）。在所有序列中，3'端添加多聚鸟苷串的CpG-ODNF 3对鸡BM-DC的刺激效果最好。综上所述，本研究首次证实了不同CpG-ODN对鸡BM-DC的成熟有不同的促进作用，其中CpG-ODN F3的促进作用最强，可作为鸡BM-DC成熟的有效刺激剂。
CpG oligonucleotide (CpG-ODN) can exert an immunostimulatory effect on different types of immune cells such as dendritic cells (DC). The immunostimulatory activity of CpG-ODN is closely related to its nucleotide sequence and structural characteristics. In this study, we aimed at evaluating the stimulatory effects of different CpG-ODN on the maturation of chicken bone marrow-derived DC (BM-DC) in vitro. First, 4 CpG-ODN were designed. Then chicken bone marrow cells were extracted from tibia and femur and cultured in the RPMI 1640 medium with recombinant chicken granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. After culture for 6 d, the cells were stimulated by different CpG-ODN or lipopolysaccharide for 24 h. Finally, the effects of different CpG-ODN on the maturation of chicken BM-DC were investigated by morphologic, phenotypic, and functional assays. The results showed that the cultured cells could display the typical DC morphology, and the CpG-ODN could efficiently stimulate the BM-DC to show the mature morphologic characteristics and upregulate the expression of cluster of differentiation (CD) 40 and CD86 molecules. In addition, after stimulation by CpG-ODN, the BM-DC could significantly induce T-cell proliferative response (P < 0.01). Among all the sequences, the stimulatory effect of CpG-ODN F3 with an addition of poly-guanosine strings at the 3' end was the best on the chicken BM-DC. In conclusion, this is the first report to demonstrate that different CpG-ODN have distinct stimulatory effects on the maturation of chicken BM-DC and CpG-ODN F3 with the best stimulatory effect can be a potent stimulant for the maturation of chicken BM-DC.