Intracellular distributions of mechanochemical proteins in cultured fibroblasts.
Intracellular distributions of mechanochemical proteins in cultured fibroblasts.
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培养的成纤维细胞中机械化学蛋白质的细胞内分布。
DOI：
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发表时间：
1977
影响因子：
11.1
通讯作者：
S. Singer
中科院分区：
文献类型：
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作者：
M. Heggeness;K. Wang;S. Singer
我们已经使用的方法，允许同时荧光染色的细胞内肌动蛋白与肌球蛋白，细丝蛋白，或微管蛋白在正常大鼠肾成纤维细胞单层培养。在细胞体的主要部分，肌动蛋白、肌球蛋白和细丝蛋白都以两种结构存在：在一种结构中，这三种蛋白质存在于相同的纤维束（应力纤维）中;在另一种结构中，这三种蛋白质呈弥散分布。然而，在部分细胞周边-在微刺的基底区，在皱褶，并在细胞与细胞接触的区域-肌动蛋白和细丝蛋白是存在的，但肌球蛋白是严重耗尽或缺席。微管以独立于应力纤维的分布存在于细胞体中，并且大多数不在细胞周边。微刺和皱褶是细胞表面上的高度动态结构，并且细胞-细胞接触的区域通常由两个接触细胞上的皱褶的缔合产生。因此，细丝蛋白和肌动蛋白而不是肌球蛋白存在于细胞表面的这些特化区域中，连同最近的证明[Wang，K. & Singer，S. J.（1977）Proc. Acad. Sci. USA 74，2021-2025）]，纯细丝蛋白与溶液中的单个F-肌动蛋白细丝相互作用以形成纤维束和片状结构，表明体内细丝蛋白-肌动蛋白相互作用在肌动蛋白细丝结构的控制、细胞运动性和细胞-细胞接触的稳定中起重要作用。
We have used methods that have allowed simultaneous fluorescent staining of intracellular actin together with either myosin, filamin, or tubulin in normal rat kidney fibroblasts in monolayer culture. In the main portions of the cell body, the actin, myosin, and filamin are all present in two structures: in one, the three proteins are present in the same fiber bundles (stress fibers); in the other, there is a diffuse distribution of the three proteins. On portions of the cell periphery however-in the basal regions of microspikes, in ruffles, and in regions of cell-cell contact-actin and filamin are present, but myosin is severely depleted or absent. Microtubules are present in the cell body in a distribution independent of the stress fibers and are mostly absent from the cell periphery. Microspikes and ruffles are highly dynamic structures on the cell surface, and regions of cell-cell contact generally result from the association of ruffles on the two contacting cells. Therefore, the presence of filamin and actin but not myosin in these specialized regions on the cell surface, together with the recent demonstration [Wang, K. & Singer, S. J. (1977) Proc. Natl. Acad. Sci. USA 74, 2021-2025)] that pure filamin interacts with individual F-actin filaments in solution to form fiber bundles and sheet-like structures, suggest that in vivo filamin-actin interactions play an important role in the control of actin filament structure, in cell motility, and in the stabilization of cell-cell contacts.