A Novel Peptide Mediates Aggregation and Migration of Hemocytes from an Insect
A Novel Peptide Mediates Aggregation and Migration of Hemocytes from an Insect
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DOI：
10.1016/j.cub.2009.03.050
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发表时间：
2009-05-12
期刊：
影响因子：
9.2
通讯作者：
Hayakawa, Yoichi
中科院分区：
文献类型：
--
作者：
Nakatogawa, Shin-ichi;Oda, Yasunori;Hayakawa, Yoichi
昆虫血细胞(血细胞)是免疫系统的重要组成部分[1-7]。血细胞识别和吞噬外来入侵者的几个因素已被确定，但调节血细胞运动的机制仍不完整。果蝇的胚胎血细胞在PVF配体的趋化信号作用下沿着常规路线迁移，PVF配体是血小板衍生生长因子家族的成员。胚胎和幼虫的血细胞也聚集在外部伤口[11-13]，但PVF不是这种反应所必需的，这表明有其他未知因素参与其中。本文报道了从菜蛾体内分离到的血细胞趋化多肽(HCP)的鉴定结果，并证明它能刺激吞噬血细胞的聚集和定向运动。时空研究表明，HCP在表皮细胞和血细胞中都有表达，而结构-功能研究发现翻译后修饰对活性很重要。HCP还与另一组来自蛾子的细胞因子有相似之处，称为ENF肽[14-17]。综上所述，我们的结果确认HCP是一种趋化细胞因子，可以增强幼虫伤口处的凝血。
Insect blood cells (hemocytes) comprise an essential arm of the immune system [1-7]. Several factors mediating recognition and phagocytosis of foreign intruders by hemocytes have been identified, but the mechanisms regulating hemocyte movement remain fragmentary. Embryonic hemocytes from Drosophila migrate along stereotypical routes in response to chemotactic signals from PVF ligands, members of the platelet-derived growth factor family [8-12]. Embryonic and larval hemocytes also accumulate at external wounds [11-13], but PVFs are not required for this response, suggesting involvement by other, unknown factors. Here we report the identification of hemocyte chemotactic peptide (HCP) from the moth Pseudaletia separata and present evidence that it stimulates aggregation and directed movement of phagocytic hemocytes. Spatiotemporal studies revealed that HCP is expressed in both epidermal cells and hemocytes, whereas structure-function studies identified post-translational modifications important for activity. HCP also shares similarities with another group of cytokines from moths called ENF peptides [14-17]. Taken together, our results identify HCP as a chemotactic cytokine that enhances clotting at wound sites in larvae.