Transcription factor hStaf/ZNF 143 is required for expression of the human TFAM gene
Transcription factor hStaf/ZNF 143 is required for expression of the human TFAM gene
复制标题
DOI：
10.1016/j.gene.2007.07.011
复制
发表时间：
2007-10-15
期刊：
影响因子：
3.5
通讯作者：
Carbon, Philippe
中科院分区：
文献类型：
--
作者：
Gerard, Marie-Aline;Krol, Alain;Carbon, Philippe
线粒体转录因子 A (Tfam) 对于线粒体 DNA 的转录起始和复制至关重要。先前报道转录因子Sp1、NRF-1、NRF-2对于维持哺乳动物TFAM基因的正常转录水平至关重要。在这项工作中，对人类 TFAM 基因转录调控的研究揭示了转录因子 hStaf/ZNF 143 存在两个跨物种保守结合位点。通过使用启动子结合测定、突变 TFAM 报告基因构建体的瞬时表达和染色质免疫沉淀实验，我们深入了解了 hStaf/ZNF143 在启动子活性中的参与。此外，我们报告了另外两个重要功能元素的识别。总而言之，我们的数据得出这样的结论：人类 TFAM 基因的启动子包含一个复杂的组织，至少有六个转录调控元件。 (c) 2007 Elsevier B.V 保留所有权利。
The mitochondrial transcription factor A (Tfam) is essential for transcription initiation and replication of mitochondrial DNA. It was previously reported that transcription factors Sp1, NRF-1, NRF-2 were critical for maintaining the normal transcription levels of the mammalian TFAM gene. In this work, investigation of the transcriptional regulation of the human TFAM gene revealed the presence of two cross-species conserved binding sites for the transcription factor hStaf/ZNF 143. By using promoter binding assays, transient expression of mutant TFAM reporter gene constructs and chromatin immunoprecipitation experiments, we provided insight into the involvement of hStaf/ZNF143 in promoter activity. Furthermore, we reported the identification of two other functionally important elements. Altogether, our data led to the conclusion that the promoter of the human TFAM gene harbors a complex organization with at least six transcriptional regulatory elements. (c) 2007 Elsevier B.V All rights reserved.