Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli
Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli
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DOI：
10.1073/pnas.96.9.4971
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发表时间：
1999-04-27
影响因子：
11.1
通讯作者：
de Boer, PAJ
中科院分区：
文献类型：
--
作者：
Raskin, DM;de Boer, PAJ
将分裂隔膜准确放置在大肠杆菌细胞的中点需要一般分裂抑制剂（MinC）、分裂抑制的位点特异性抑制剂（MinE）和ATP酶（MinD）的联合作用，这是MinC和MinE正常发挥功能所必需的。我们先前表明，功能性MinE-GFP融合物在细胞中部/附近的环状结构中积累。在这里，我们表明，功能GFP-MinD积累交替在任何一个细胞的一半，似乎是一个快速振荡的膜缔合解离周期所施加的MinE。结果表明，MinD代表了细菌中一种新型的动态细胞元件，在将分裂装置引导到细胞中间方面具有多种作用。
Accurate placement of the division septum at the midpoint of Escherichia coli cells requires the combined action of a general division inhibitor (MinC), a site-specific suppressor of division inhibition (MinE), and an ATPase (MinD) that is required for proper functioning of both MinC and MinE. We previously showed that a functional MinE-Gfp fusion accumulates in a ring structure at/near the middle of cells. Here we show that functional Gfp-MinD accumulates alternately in either one of the cell halves in what appears to be a rapidly oscillating membrane association-dissociation cycle imposed by MinE. The results indicate that MinD represents a novel type of dynamic cellular element in bacteria, with multiple roles in directing the division apparatus to the middle of the cell.