Temporally and Genetically Discrete Periods of Wheat Sensitivity to High Temperature.
Temporally and Genetically Discrete Periods of Wheat Sensitivity to High Temperature.
复制标题
在时间和基因上离散的小麦对高温的敏感性。
DOI：
10.3389/fpls.2017.00051
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发表时间：
2017
影响因子：
5.6
通讯作者：
Gooding MJ
中科院分区：
文献类型：
--
作者：
Barber HM;Lukac M;Simmonds J;Semenov MA;Gooding MJ
连续一天将盆栽小麦转移到高温(35/30℃昼夜)可重复控制的环境中，从第二节可检测到乳熟期，提供了最有力的证据，表明小麦的育性在两个离散的敏感高峰期非常容易受到高温胁迫的影响：早期孕穗期[十进制生长期(GS)41-45]和开花早期(GS 61-65)。对两个不同的优良品系(塞尔维亚上市的Renesansa，PPD-D1a，Rht8；英国上市的Savannah，PPD-D1b，Rht-D1b)的粒数和粒重数据同时进行了双高斯拟合，确定了主茎感病高峰期集中在3(S.E.=0.82)和18(S.E.=0.55天(日平均气温=14.3°C)。影响的严重程度取决于基因型、生育期及其互作：孕穗期萨凡纳和开花期瑞尼萨的籽粒结实率相对于20/15℃的籽粒结实率下降到80%以下。萨凡纳对开花期的高温胁迫具有较强的耐受性。进一步的实验包括62个作图株系，Renesansa×Savannah的加倍单倍体后代，发现开花期耐性与PPD-D1b，Rht-D1b有关，并在2A染色体上发现了来自Renesansa的QTL。在启动过程中，没有一个相关的标记与耐受性有关。Rht8与热应激耐受性从来没有联系，这一点在进一步的实验中得到了证实，该实验将Rht8包括在一个cv中的近等基因系的比较中。Paragon背景。平均粒重有一定的补偿性增加，但只有在孕穗期间施加胁迫和没有PPD-D1a的情况下才能观察到。
Successive single day transfers of pot-grown wheat to high temperature (35/30°C day/night) replicated controlled environments, from the second node detectable to the milky-ripe growth stages, provides the strongest available evidence that the fertility of wheat can be highly vulnerable to heat stress during two discrete peak periods of susceptibility: early booting [decimal growth stage (GS) 41–45] and early anthesis (GS 61–65). A double Gaussian fitted simultaneously to grain number and weight data from two contrasting elite lines (Renesansa, listed in Serbia, Ppd-D1a, Rht8; Savannah, listed in UK, Ppd-D1b, Rht-D1b) identified peak periods of main stem susceptibility centered on 3 (s.e. = 0.82) and 18 (s.e. = 0.55) days (mean daily temperature = 14.3°C) pre-GS 65 for both cultivars. Severity of effect depended on genotype, growth stage and their interaction: grain set relative to that achieved at 20/15°C dropped below 80% for Savannah at booting and Renesansa at anthesis. Savannah was relatively tolerant to heat stress at anthesis. A further experiment including 62 lines of the mapping, doubled-haploid progeny of Renesansa × Savannah found tolerance at anthesis to be associated with Ppd-D1b, Rht-D1b, and a QTL from Renesansa on chromosome 2A. None of the relevant markers were associated with tolerance during booting. Rht8 was never associated with heat stress tolerance, a lack of effect confirmed in a further experiment where Rht8 was included in a comparison of near isogenic lines in a cv. Paragon background. Some compensatory increases in mean grain weight were observed, but only when stress was applied during booting and only where Ppd-D1a was absent.