MACRORESTRICTION MAPPING OF COL4A1 AND COL4A2 COLLAGEN GENES ON HUMAN-CHROMOSOME 13Q34
MACRORESTRICTION MAPPING OF COL4A1 AND COL4A2 COLLAGEN GENES ON HUMAN-CHROMOSOME 13Q34
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DOI：
10.1016/0888-7543(88)90086-9
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发表时间：
1988-10-01
期刊：
影响因子：
4.4
通讯作者：
FRANCOMANO, CA
中科院分区：
文献类型：
--
作者：
CUTTING, GR;KAZAZIAN, HH;FRANCOMANO, CA
α基因1和α- IV型胶原的2条链（COL 4A 1和COL 4A 2）映射到相同的染色体带（13 q34），并具有高度的核苷酸同源性。我们已经使用脉冲场凝胶电泳和克隆COL 4A 1和COL 4A 2 DNA片段作为分子探针，构建一个1200 kb的宏观限制性内切酶图谱，其中包括这两个基因。这两个基因位于一个340 kb的区域内，其中COL 4A 2的3“末端和COL 4A 1的5”区域相隔至少100 kb但不超过160 kb。因此，这些基因是染色体13 q34上基因簇的两个成员。
The genes for the .alpha.-1 and .alpha.-2 chains of type IV collagen (COL4A1 and COL4A2) map to the same chromosomal band (13q34) and have a high degree of nucleotide homology. We have used pulsed field gel electrophoresis and cloned COL4A1 and COL4A2 DNA fragments as molecular probes to construct a 1200-kb macrorestriction map which encompasses both genes. The two genes are located within a 340-kb region with the 3'' end of COL4A2 and the 5'' region of COL4A1 separated by at least 100 kb but not more than 160 kb. These genes, therefore, are two members of a gene cluster on chromosome 13q34.