An electrochemical DNA biosensor for highly sensitive detection of phosphinothricin acetyltransferase gene sequence based on polyaniline-(mesoporous nanozirconia)/poly-tyrosine film
An electrochemical DNA biosensor for highly sensitive detection of phosphinothricin acetyltransferase gene sequence based on polyaniline-(mesoporous nanozirconia)/poly-tyrosine film
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DOI：
10.1016/j.snb.2011.12.064
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发表时间：
2012-02
影响因子：
8.4
通讯作者：
Jie Yang;Xueliang Wang;Huaqiang Shi
中科院分区：
文献类型：
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作者：
Jie Yang;Xueliang Wang;Huaqiang Shi
基于聚苯胺-（介孔纳米氧化锆）/聚酪氨酸膜修饰玻碳电极（GCE），建立了一种高灵敏检测DNA杂交和转基因植物筛选检测基因之一的草丁膦乙酰转移酶（PAT）基因片段的方法。该膜具有良好的固定DNA探针（ss-DNA）和传感DNA杂交的性能。利用循环伏安法（CV）和电化学阻抗谱（EIS）研究了该膜的制备过程、DNA在膜上的固定和杂交，发现电极表面的电子传递电阻（Ret）随与DNA探针杂交的靶DNA浓度的增加而增加，可用于DNA杂交和PAT基因的检测。动态检测范围为1.0×10 - 13 ~ 1.0×10 - 6 molL-1，检出限为2.68×10 - 14 molL-1。与其他基于氧化锆基材料的电化学DNA生物传感器相比，该传感器具有结构简单、稳定性好、选择性好、灵敏度高等特点。
A highly sensitive method for detection of DNA hybridization and phosphinothricin acetyltransferase (PAT) gene fragment, one of the screening detection genes of the transgenic plants, was developed based on the glassy carbon electrode (GCE) modified with polyaniline-(mesoporous nanozirconia)/poly-tyrosine film. The film exhibited excellent properties for immobilizing DNA probes (ss–DNA) and sensing DNA hybridization. The fabrication processes of the film, the DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), and found that the electron transfer resistance (Ret) of the electrode surface increased with the concentration of the target DNA hybridized with the DNA probes, which could be applied for determination of the DNA hybridization and PAT gene. The dynamic detection range was from 1.0×10−13molL−1to 1.0×10−6molL−1, and the detection limit was 2.68×10−14molL−1. Compared with the other electrochemical DNA biosensors based on zirconia-based materials, the proposed biosensor showed its own performance of simplicity, good stability, fine selectivity and high sensitivity.