Expression of cytochrome P450 cholesterol side chain cleavage and 3beta-hydroxysteroid dehydrogenase during embryogenesis in chicken adrenal glands and gonads.
Expression of cytochrome P450 cholesterol side chain cleavage and 3beta-hydroxysteroid dehydrogenase during embryogenesis in chicken adrenal glands and gonads.
复制标题
鸡肾上腺和性腺胚胎发生过程中细胞色素 P450 胆固醇侧链裂解和 3β-羟基类固醇脱氢酶的表达。
DOI：
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发表时间：
2000
影响因子：
2.7
通讯作者：
T. Mori
中科院分区：
文献类型：
--
作者：
I. Kanda;Y. Akazome;O. Ogasawara;T. Mori
在孵化第 4 天至第 12 天期间，检查鸡胚肾上腺和性腺中细胞色素 P450 胆固醇侧链裂解 (P450scc) 和 3β-羟基类固醇脱氢酶 (3β-HSD) mRNA 的表达。原位杂交分析显示，3β-HSD mRNA 在孵育第 5 天在肾上腺中出现，在第 6 天在性腺中出现，而 P450scc mRNA 在孵育第 7 天在肾上腺和性腺中都有表达。表达这两种酶 mRNA 的细胞分布在肾上腺的类固醇生成组织和性腺的髓索中。从孵育第9天到第11天，在大部分肾上腺和性腺中均未发现P450scc mRNA表达，但在第12天再次在两者中检测到，尽管在此期间3β-HSD mRNA持续表达。 P450scc mRNA 表达模式的变化与先前报道的血浆皮质酮水平的变化平行。因此，表明P450scc对于胚胎发生至关重要。
Expression of cytochrome P450 cholesterol side chain cleavage (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) mRNAs was examined in chicken embryonic adrenal glands and gonads between days 4 and 12 of incubation. In situ hybridization analysis showed that 3beta-HSD mRNA appeared on day 5 of incubation in the adrenal glands and on day 6 in the gonads, while P450scc mRNA was expressed on day 7 in both the adrenal glands and the gonads. Cells expressing both enzyme mRNAs were distributed in the steroidogenic tissues of the adrenal glands and in the medullary cords of the gonads. From days 9 to 11 of incubation, P450scc mRNA expression was not found in the majority of both the adrenal glands and the gonads, but was detected again in both on day 12, although 3beta-HSD mRNA was constitutively expressed during this period. Changes in the expression pattern of P450scc mRNA are paralleled by changes in the plasma corticosterone level reported previously. Therefore, it is suggested that P450scc is essential to embryogenesis.
影响因子：
4.8
作者：
Coulter,CL;Goldsmith,PC;Mesiano,S;Voytek,CC;Martin,MC;Mason,JI;Jaffe,RB
通讯作者：
Jaffe,RB
推导鸡胆固醇侧链裂解细胞色素P450的血红素结合区的氨基酸序列。
DOI：
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发表时间：
1990
期刊：
Protein sequences & data analysis
影响因子：
--
作者：
Mathew,PA;Kagawa,N;Bhasker,CR;Waterman,MR
通讯作者：
Waterman,MR