EFFECTS OF LIGHT AND LOW-TEMPERATURES ON CHLOROPHYLL CONTENT AND METABOLISM OF CHLORELLA-SOROKINIANA SHIHIRA AND KRAUSS
EFFECTS OF LIGHT AND LOW-TEMPERATURES ON CHLOROPHYLL CONTENT AND METABOLISM OF CHLORELLA-SOROKINIANA SHIHIRA AND KRAUSS
复制标题
DOI：
10.1016/0098-8472(79)90025-x
复制
发表时间：
1979-01-01
影响因子：
5.7
通讯作者：
KARLANDER, EP
中科院分区：
文献类型：
--
作者：
SPEARING, AM;KARLANDER, EP
Marvin和Karlander发现，亚适宜温度诱导的C. sorokiniana叶绿素白化需要光和分子O2。对不同光质量和光量的研究表明，漂白依赖于光强，是一个两阶段的过程。白色荧光灯在降解叶绿素方面效率最高，然后依次是蓝光、白炽灯和红光。低强度蓝光（2.55-4.0 mW/cm2）和红光（2.8 mW/cm2）比高强度蓝光或红光产生叶绿素的初始下降更快。低强度蓝光和红光曲线也表现出持续100 h以上的中期高原。在低强度荧光和白炽光下没有出现中期高原。在10℃时，O2析出率随孵育时间的增加而下降。C. 4.0 mW/cm2蓝光的破坏性最小，使O2的演化时间延长了近4倍。温度高于22度的细胞。C在39℃时的光合作用和呼吸作用模式接近对照细胞。C.温度低于22度的细胞孵育。C显示的模式接近于在10度漂白的细胞。C.在叶绿素大量流失之前，Hill活性在初始阶段被破坏。在加速白化阶段，细胞活力与叶绿素的损失密切相关。
Chlorophyll bleaching induced by suboptimal temperatures in C. sorokiniana was shown by Marvin and Karlander to require light and molecular O2. This study with various light qualities and quantities showed that bleaching was light intensity dependent and a 2-phase process. White fluorescent light was the most efficient in degrading chlorophyll, then in order, blue, incandescent white and red. Low intensity blue, 2.55-4.0 mW(watts)/cm2, and red light, 2.8 mW/cm2, produced an initial decline in chlorophyll more rapid than higher intensities of blue or red light. The low intensity blue and red light curve also showed a mid time-course plateau lasting beyond 100 h. No mid time-course plateau occurred at low intensities of luorescent or incandescent white light. O2 evolution declined with increasing time of incubation at 10.degree. C. Blue light of 4.0 mW/cm2 was least destructive, extending the duration of O2 evolution to nearly 4 times that obtained with white light. Cells incubated at temperatures greater than 22.degree. C exhibited patterns of photosynthesis and respiration approaching those of control cells at 39.degree. C. Cells incubated at temperatures less than 22.degree. C showed patterns approaching those of cells subjected to bleaching at 10.degree. C. Hill activity was destroyed during the initial phase before much loss of chlorophyll had occurred. Viability of cells was more closely associated with loss of chlorophyll in the accelerated bleaching phase.