Point-of-need detection of microcystin-LR using a smartphone-controlled electrochemical analyzer
Point-of-need detection of microcystin-LR using a smartphone-controlled electrochemical analyzer
复制标题
使用智能手机控制的电化学分析仪对微囊藻毒素-LR 进行按需检测
DOI：
10.1016/j.snb.2019.05.028
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发表时间：
2019-09-01
影响因子：
8.4
通讯作者：
Lei, Hongtao
中科院分区：
文献类型：
--
作者：
Guan, Tian;Huang, Wenzheng;Lei, Hongtao
微囊藻毒素-LR（MC-LR）的快速、原位检测方法的发展具有便携、可靠、使用方便等特点，但目前还存在不足。在这项研究中，一个便携式和无线点的需要检测系统的微囊藻毒素-LR（MC-LR）的定量开发和验证。整个系统由三个主要部分组成：丝网印刷碳电极（SPCE），智能手机控制的电化学分析仪（SCEA）和Android的智能手机。SCEA是一台国产的基于ARM STM 32微处理器的电化学分析仪，与商用电化学工作站相比具有相似的性能。安装有用户友好应用程序的智能手机用于控制分析仪，接收和分析数据，并实时显示检测结果。该系统可以准确地定量MC-LR在0.001-100 μ g/L的范围内，检测限为0.00011 μ g/L，结果与LC-MS/MS一致。此外，通过测量位于珠江三角洲（中国广东省）的6个水库的水样，使用该基于智能手机的平台生成了初步的MC-LR污染地图。本文报道的基于智能手机的检测系统具有成本效益、可靠性和易用性，可以促进活性毒物筛查，以保证饮用水的安全，特别是在资源有限的地区。
The development of rapid and in situ detection method for microcystin-LR (MC-LR) with portable, reliable, and easy to use devices are highly demanded, but it is still deficient. In this study, a portable and wireless point-of-need detection system for microcystin-LR (MC-LR) quantitation was developed and validated. The whole system consisted of three main parts: screen printed carbon electrodes (SPCEs), smartphone-controlled electrochemical analyzer (SCEA), and Android's smartphone. The SCEA was a homemade ARM STM32 microcontroller-based electrochemical analyzer, which have similar performance compared with a commercially available electrochemical workstation. A smartphone installed with a user-friendly application was employed to control the analyzer, receive and analyze data, and display detection results in real-time. This system can accurately quantitate MC-LR in the range of 0.001-100 mu g/L with a detection limit of 0.00011 mu g/L, and the results were consistent with LC-MS/MS. In addition, a preliminary MC-LR contamination map was generated using this smartphone-based platform by measuring water samples at 6 reservoirs located in Pearl River Delta (Guangdong Province, China). The cost-effective, reliable and easy-to-use smartphone-based detection system reported here can facilitate active toxicant screening to guarantee the safety of the drinking water, particularly in resource-limited area.