Insight into the Unique Fluorescence Quenching Property of Metal-Organic Frameworks upon DNA Binding
Insight into the Unique Fluorescence Quenching Property of Metal-Organic Frameworks upon DNA Binding
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深入了解金属有机框架在 DNA 结合时独特的荧光猝灭特性
DOI：
10.1021/acs.analchem.7b02256
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发表时间：
2017
影响因子：
7.4
通讯作者：
Chen Hong-Yuan
中科院分区：
文献类型：
--
作者：
Wang Huai-Song;Liu Hai-Ling;Wang Kang;Ding Ya;Xu Jing-Juan;Xia Xing-Hua;Chen Hong-Yuan
金属有机骨架（MOFs）已成功地用作荧光DNA检测的有效猝灭剂。然而，MOFs的表面电荷性质不可避免地会影响其荧光猝灭行为。本文中，纳米级MOFs（NMOFs），包括MOFs纳米片和纳米颗粒，已经被用于研究NMOFs的荧光猝灭与表面性质之间的关系。我们发现，带正电荷和负电荷的NMOFs表现出完全相反的荧光猝灭性质对带负电荷的FAM标记的双链DNA（dsDNA）。相反，它们对带正电荷的TAMRA标记的dsDNA的感测显示出可忽略的影响。该研究为NMOFs的荧光猝灭特性提供了新的认识，为构建比率荧光DNA生物传感器提供了新的思路。
Metal-organic frameworks (MOFs) have been successfully used as efficient quenchers for fluorescent DNA detection. However, the surface charge property of MOFs can inevitably affect their fluorescence quenching behavior. Herein, nanoscale MOFs (NMOFs), including MOF nanosheets and nanoparticles, have been employed to investigate the relationship between the fluorescence quenching and surface properties of NMOFs. We find that the positively and negatively charged NMOFs exhibited totally opposite fluorescence quenching properties toward negatively charged FAM-labeled double-stranded DNA (dsDNA). On the contrast, they show negligible influence on the sensing of positively charged TAMRA-labeleddsDNA. This study provides a new insight of the fluorescence quenching property of NMOFs and offers a new concept for construction of ratiometric fluorescence DNA biosensors.