Dopamine terminals in the rat prefrontal cortex synapse on pyramidal cells that project to the nucleus accumbens
Dopamine terminals in the rat prefrontal cortex synapse on pyramidal cells that project to the nucleus accumbens
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DOI：
10.1523/jneurosci.19-24-11049.1999
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发表时间：
1999-12-15
影响因子：
5.3
通讯作者：
Sesack, SR
中科院分区：
文献类型：
--
作者：
Carr, DB;O'Donnell, P;Sesack, SR
腹侧被盖区多巴胺神经元对前额叶皮层的传入与工作记忆过程和精神分裂症的发病机制有关。以前的解剖学研究表明，多巴胺末梢突触树突棘和轴的锥体细胞在PFC。此外，神经化学和生理学研究表明，多巴胺调节PFC神经元的活动，项目的延髓核。然而，这种调制是否涉及直接突触输入到皮质-突触投射神经元尚未确定。为了解决这个问题，将伪狂犬病病毒（PRV）减毒株从延髓核的逆行转运与酪氨酸羟化酶（TH）的免疫过氧化物酶标记相结合，以鉴定PFC中的多巴胺末梢。
Afferents to the prefrontal cortex (PFC) from dopamine neurons in the ventral tegmental area have been implicated in working memory processes and in the pathogenesis of schizophrenia. Previous anatomical investigations have demonstrated that dopamine terminals synapse on dendritic spines and shafts of pyramidal cells in the PFC. Moreover, neurochemical and physiological studies suggest that dopamine modulates the activity of PFC neurons that project to the nucleus accumbens. However, whether this modulation involves direct synaptic input to cortico-accumbens projection neurons has not been determined. To address this question, retrograde transport of an attenuated strain of pseudorabies virus (PRV) from the nucleus accumbens was combined with immunoperoxidase labeling of tyrosine hydroxylase (TH) to identify dopamine terminals in the PFC. At survival times