Transcriptomics assisted proteomic analysis of Nicotiana occidentalis infected by Candidatus Phytoplasma mali strain AT
Transcriptomics assisted proteomic analysis of Nicotiana occidentalis infected by Candidatus Phytoplasma mali strain AT
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DOI：
10.1002/pmic.201300551
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发表时间：
2014-08-01
期刊：
影响因子：
3.4
通讯作者：
Sauer, Sascha
中科院分区：
文献类型：
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作者：
Luge, Toni;Kube, Michael;Sauer, Sascha
植原体是软体动物纲的致病菌，与 1000 多种植物病害有关。在本研究中，我们应用定量质谱分析了模型植物烟草（Nicotiana occidentalis）在 Candidatus Phytoplasma mali 菌株 AT 感染后受影响的途径。利用从叶中脉获得的组织，鉴定出了 1466 种植物相关蛋白。对于这些蛋白质中的 1019 个，我们可以重复地量化受感染植物与未受感染植物的表达变化，其中 157 个蛋白质上调，173 个蛋白质下调。差异表达发生在许多途径中，其中包括卟啉和叶绿素代谢的强烈下调以及α-亚麻酸代谢的上调，这与观察到的植物防御关键信号分子茉莉酸水平的增加一致。我们的数据揭示了参与植物防御植原体感染的分子网络，并为进一步研究提供了资源。
Phytoplasmas are pathogenic bacteria within the class of Mollicutes, which are associated with more than 1000 plant diseases. In this study, we applied quantitative mass spectrometry to analyse affected pathways of the model plant tobacco (Nicotiana occidentalis) upon Candidatus Phytoplasma mali strain AT infection. Using tissue obtained from leaf midribs, 1466 plant-assigned proteins were identified. For 1019 of these proteins, we could reproducibly quantify the expression changes of infected versus noninfected plants, of which 157 proteins were up-and 173 proteins were downregulated. Differential expression took place in a number of pathways, among others strong downregulation of porphyrin and chlorophyll metabolism and upregulation of alpha-linolenic acid metabolism, which was consistent with observed increased levels of jasmonic acid, a key signal molecule of plant defence. Our data shed light on the molecular networks that are involved in defence of plants against phytoplasma infection and provide a resource for further studies.