Alphaviral vectors for gene transfer into neurons
Alphaviral vectors for gene transfer into neurons
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DOI：
10.1385/mn:26:2-3:183
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发表时间：
2002-10-01
影响因子：
5.1
通讯作者：
Ehrengruber, MU
中科院分区：
文献类型：
--
作者：
Ehrengruber, MU
甲病毒是小型有包膜的正链 RNA 病毒，已成功转化为表达载体，如塞姆利基森林病毒 (SFV)、辛德比斯病毒 (SIN) 和委内瑞拉马脑炎病毒。与其他病毒载体相比，其优点是重组病毒颗粒生成简单、快速、起效快、转基因表达水平高。当应用于神经元组织时，SFV 和 SIN 载体具有高效且优先转导神经元而不是非神经元细胞的额外优势。本文概述了 SFV 和 SIN 的生物学、它们生成表达载体以及它们在神经生物学中的应用，特别强调海马神经元的转导。此外，它描述了α病毒载体的最新发展，其具有降低或不存在细胞毒性和降低的转基因表达、温度可控基因表达以及中枢神经系统(CNS)中改变的宿主细胞特异性。最后，该综述评估了 SFV 和 SIN 载体与重组慢病毒、5 型腺病毒、2 型腺相关病毒和麻疹病毒在海马组织培养中的使用。
Alphaviruses are small, enveloped positive-strand RNA viruses that have been successfully transformed into expression vectors in the case of Semliki Forest virus (SFV), Sindbis virus (SIN), and Venezuelan equine encephalitis virus. Compared to other viral vectors, their advantages are easy and fast generation of recombinant viral particles, rapid onset, and high-level transgene expression. When applied to neuronal tissue, SFV and SIN vectors possess the additional advantage of efficiently and preferentially transducing neurons rather than non-neuronal cells. This article gives an overview of the biology of SFV and SIN, their generation into expression vectors, and their application in neurobiology, with particular emphasis on the transduction of hippocampal neurons. hi addition, it describes the more recent development of alphaviral vectors with decreased or absent cytotoxicity and lowered transgene expression, temperature-controllable gene expression, and altered host-cell specificity in the central nervous system (CNS). Finally, the review evaluates the use of SFV and SIN vectors in hippocampal tissue cultures vs recombinant lentivirus, adenovirus type 5, adeno-associated virus type 2, and measles virus.