Eco1-dependent cohesin acetylation anchors chromatin loops and cohesion to define functional meiotic chromosome domains.
Eco1-dependent cohesin acetylation anchors chromatin loops and cohesion to define functional meiotic chromosome domains.
复制标题
DOI：
10.7554/elife.74447
复制
发表时间：
2022-02-01
期刊：
影响因子：
7.7
通讯作者：
Marston AL
中科院分区：
文献类型：
--
作者：
Barton RE;Massari LF;Robertson D;Marston AL
粘着蛋白通过形成染色体内环和姐妹染色单体间连接来组织基因组。在减数分裂形成配子的过程中，染色体被重塑以支持交叉重组和连续两轮的染色体分离。在这里，我们表明，减数分裂染色体组织成功能域的Eco1乙酰转移酶依赖定位的染色质环和姐妹染色单体凝聚力在芽殖酵母。Eco1乙酰化Smc3的凝聚素亚基在减数分裂S期建立染色质边界，独立的DNA复制。边界形成的Eco1是至关重要的前期出口和维持凝聚力，直到减数分裂II，但独立的Eco1拮抗的凝聚素释放因子，Wpl1的能力。相反，Wpl1阻止粘着蛋白释放对于减数分裂I中的着丝粒粘着、动粒单取向和姐妹染色单体的共分离是必不可少的。我们的研究结果建立Eco1作为一个关键的决定因素的染色质边界和凝聚力的定位，揭示了如何本地染色体结构指导基因组传输到配子。
Cohesin organizes the genome by forming intra-chromosomal loops and inter-sister chromatid linkages. During gamete formation by meiosis, chromosomes are reshaped to support crossover recombination and two consecutive rounds of chromosome segregation. Here, we show that meiotic chromosomes are organized into functional domains by Eco1 acetyltransferase-dependent positioning of both chromatin loops and sister chromatid cohesion in budding yeast. Eco1 acetylates the Smc3 cohesin subunit in meiotic S phase to establish chromatin boundaries, independently of DNA replication. Boundary formation by Eco1 is critical for prophase exit and for the maintenance of cohesion until meiosis II, but is independent of the ability of Eco1 to antagonize the cohesin release factor, Wpl1. Conversely, prevention of cohesin release by Wpl1 is essential for centromeric cohesion, kinetochore mono-orientation, and co-segregation of sister chromatids in meiosis I. Our findings establish Eco1 as a key determinant of chromatin boundaries and cohesion positioning, revealing how local chromosome structuring directs genome transmission into gametes.