Detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects
Detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects
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DOI：
10.1111/j.1399-302x.2004.00172.x
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发表时间：
2004-12-01
影响因子：
--
通讯作者：
Takahashi, N
中科院分区：
文献类型：
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作者：
Mayanagi, G;Sato, T;Takahashi, N
本研究的目的是比较 18 名未经治疗的牙周炎受试者和 12 名牙周健康受试者的龈下和龈上菌斑中 25 种细菌的检测频率。从龈下和龈上菌斑样本中提取基因组DNA，并通过16S rRNA基因的聚合酶链反应进行细菌检测。 14种细菌与牙周炎无关，这14种细菌中有11种在牙周炎和健康受试者的龈下菌斑中频繁检出（大于或等于50%）。隐真杆菌、中间普氏菌、齿垢密螺旋体等9种细菌似乎与牙周炎有关；牙周炎患者龈下菌斑样本中的检出频率高于健康受试者，但通过多重比较，这些差异并不具有统计学意义（0.002小于或等于P
The aim of this study was to compare the detection frequencies of 25 bacterial species in subgingival and supragingival plaque of 18 untreated periodontitis subjects and 12 periodontally healthy subjects. Genomic DNA was extracted from subgingival and supragingival plaque samples, and bacterial detection was performed by polymerase chain reaction of the 16S rRNA genes. Fourteen bacteria showed no relationship with periodontitis, and 11 of these 14 species were frequently detected (greater than or equal to50%) in subgingival plaque in both periodontitis and healthy subjects. Nine bacteria such as Eubacterium saphenum, Prevotella intermedia, and Treponema denticola seemed to be related to periodontitis; their detection frequencies in subgingival plaque samples were higher in periodontitis than in healthy subjects, but these differences were not statistically significant by multiple comparisons (0.002less than or equal toP