Adsorption of olive leaf (Olea europaea L.) antioxidants on silk fibroin

Adsorption of olive leaf (Olea europaea L.) antioxidants on silk fibroin
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DOI:
10.1021/jf062829o
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发表时间:
2007-02-21
影响因子:
6.1
通讯作者:
Bayraktar, Oguz
Bayraktar, Oguz
中科院分区:
农林科学1区
文献类型:
--
作者:
Baycin, Deniz;Altiok, Evren;Bayraktar, Oguz

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在不同温度、pH 值和固液比下评估了橄榄苦苷和芦丁的吸附等温线。吸附等温线的实验数据很好地拟合了Langmuir模型。最大吸附容量测定为108mg橄榄苦苷/g丝素蛋白和21mg芦丁/g丝素蛋白。吸附橄榄苦苷和芦丁后,丝素蛋白的抗氧化能力从1.93 mmol TEAC/g提高到3.61 mmol TEAC/g。丝素蛋白在吸附橄榄叶抗氧化剂后还获得了对金黄色葡萄球菌和肺炎克雷伯菌的抗菌活性。在解吸过程中,81%的芦丁和85%的橄榄苦苷在70%的乙醇水溶液中从吸附剂表面去除。因此,丝素蛋白被发现是一种有前途的生物材料,可用于生产功能性食品或膳食补充剂以及从橄榄叶提取物中纯化橄榄苦苷和芦丁。
The adsorption isotherms of oleuropein and rutin were evaluated at different temperatures, pH values, and solid/liquid ratios. The experimental data of adsorption isotherms were well fitted to a Langmuir model. The maximum adsorption capacities were determined as 108 mg of oleuropein/g of silk fibroin and 21 mg of rutin/g of silk fibroin. After adsorption of oleuropein and rutin, the antioxidant capacity of silk fibroin increased from 1.93 to 3.61 mmol of TEAC/g. Silk fibroin also gained antimicrobial activity against Staphylococcus aureus and Klebsiella pneumoniae after adsorption of olive leaf antioxidants. In a desorption process, 81% of rutin and 85% of oleuropein were removed from the adsorbent surface in 70% aqueous ethanol solution. Consequently, silk fibroin was found to be a promising biomaterial for the production of functional food or dietary supplements and for the purification of oleuropein and rutin from olive leaf extracts.