Melatonin retards senescence via regulation of the electron leakage of postharvest white mushroom (Agaricus bisporus)
Melatonin retards senescence via regulation of the electron leakage of postharvest white mushroom (Agaricus bisporus)
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褪黑素通过调节采后白蘑菇（双孢蘑菇）的电子泄漏来延缓衰老
DOI：
10.1016/j.foodchem.2020.127833
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发表时间：
2021-03-15
期刊：
影响因子：
8.8
通讯作者：
Yu, Shaoxuan
中科院分区：
文献类型：
--
作者：
Li, Ling;Kitazawa, Hiroaki;Yu, Shaoxuan
目前，褪黑激素（N-乙酰基-5-甲氧基色胺）被认为是一种潜在的自由基清除剂。在这项研究中，在3 +/- 1 ℃下评估了不同浓度（0.05、0.1和0.2 mM）的外源褪黑激素对白色蘑菇的质地、感官品质和电子泄漏的影响。观察到用0.1mM褪黑激素处理的蘑菇具有良好的质量，并且它们的电子泄漏被显著抑制。结果表明，0.1 mM褪黑激素保留了较高的腺苷三磷酸水平，也阻止了细胞色素c释放到细胞质中。更重要的是，它显着抑制电子泄漏，通过增加活动的复合物I和III的上调AbNdufB 9和AbRIP 1。它还调节蘑菇的呼吸状态;延缓呼吸状态3的下降;增强呼吸状态4;促进线粒体的氧化磷酸化和效率;并最终延缓白色蘑菇的衰老。
Currently, melatonin (N-acetyl-5-methoxytrytamine) is recognized as a potential scavenger of free radicals. In this study, the effect of exogenous melatonin at various concentrations (0.05, 0.1, and 0.2 mM) on the texture, sensory qualities, and electron leakage in white mushrooms was evaluated at 3 +/- 1 degrees C. It was observed that mushrooms treated with 0.1 mM melatonin were of good quality and their electron leakage was dramatically dampened. The results showed that 0.1 mM melatonin retained a higher adenosine triphosphate level and also prevented the release of cytochrome c into the cytoplasm. More significantly, it prominently inhibited electron leakage by increasing the activities of complexes I and III by the upregulation of AbNdufB9 and AbRIP1. It also regulated respiratory states in mushrooms; delayed the decline of respiratory state 3; enhanced respiratory state 4; boosted the oxidative phosphorylation and efficiency of mitochondria; and ultimately retarded the senescence of the white mushrooms.