Ultra-high performance liquid chromatographic determination of antioxidants in teas using inkjet-printed graphene-polyaniline electrode
Ultra-high performance liquid chromatographic determination of antioxidants in teas using inkjet-printed graphene-polyaniline electrode
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DOI：
10.1016/j.talanta.2015.05.020
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发表时间：
2016-02-01
期刊：
影响因子：
6.1
通讯作者：
Chailapakul, Orawon
中科院分区：
文献类型：
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作者：
Bardpho, Chayanee;Rattanarat, Poomrat;Chailapakul, Orawon
建立了超高效液相色谱-新型喷墨印刷导电墨水修饰电极联用技术，用于多酚类抗氧化剂的快速、同时测定。选择两种印刷技术用于制造和修改，分别包括（i）内部丝网印刷法和（ii）喷墨印刷法。采用Dimatix喷墨打印机将石墨烯/聚苯胺纳米复合材料（G-PANI）导电油墨精确地涂敷在丝网印刷碳电极（SPCE）表面。与裸SPCE相比，G-PANI修饰的丝网印刷碳电极（G-PANI/SPCE）具有更高的电化学灵敏度，每种抗氧化剂的峰电流都增加了2-4倍。此外，4种抗氧化剂成功地分离和测定3分钟内使用反相超高效液相色谱法（UHPLC）与移动的流动相含有磷酸盐缓冲液和乙腈（90：10 v/v）。在+1.2 V vs. Ag/AgCl的最佳检测电位下，抗氧化剂的线性校准和检测限（S/N=3）分别为0.01-10 μ g mL（-1）和1.38-1.94 ng mL（-1）。最后，该方法已成功地用于茶叶样品中抗氧化剂的测定，从我们提出的方法获得的结果是在一个高度良好的协议，从标准的超高效液相色谱-紫外方法获得的。(C)2015 Elsevier B. V.版权所有。
A development of ultra-high performance liquid chromatographic coupled with a novel inkjet-printed conductive ink-modified electrode for a fast and simultaneous determination of polyphenolic antioxidants was achieved. Two printing techniques were selected for fabrication and modification including (i) an in-house screen-printing method and (ii) an inkjet-printing method, respectively. A conductive ink containing graphene and polyaniline nanocomposite (G-PANI) was precisely casted onto the surface of screen-printed carbon electrode (SPCE) using a dimatix inkjet material printer. Compared to a bare SPCE, the G-PANI-modified screen-printed carbon electrode (G-PANI/SPCE) exhibited higher electrochemical sensitivity with increase (2-4 times) of peak current of each antioxidant. Moreover, four antioxidants were successfully separated and determined within 3 min using a reverse phase ultra-high performance liquid chromatography (UHPLC) with a mobile phase containing phosphate buffer and acetonitrile (90:10 v/v). Under an optimal detection potential at +1.2 V vs. Ag/AgCl, linear calibrations and limits of detection (S/N=3) for antioxidants were found to be 0.01-10 mu g mL(-1) and 1.38-1.94 ng mL(-1), respectively. Finally, this proposed method has been successfully used for the determination of antioxidants in tea samples, the results obtained from our presented method were within a highly good agreement those obtained from a standard UHPLC-UV method. (C) 2015 Elsevier B.V. All rights reserved.