BcMctA, a putative monocarboxylate transporter, is required for pathogenicity in Botrytis cinerea
BcMctA, a putative monocarboxylate transporter, is required for pathogenicity in Botrytis cinerea
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DOI：
10.1007/s00294-015-0474-1
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发表时间：
2015-11-01
期刊：
影响因子：
2.5
通讯作者：
Zhu, Tingheng
中科院分区：
文献类型：
--
作者：
Cui, Zhifeng;Gao, Nana;Zhu, Tingheng
单羧酸转运体在哺乳动物代谢中起中心作用，但在植物病原真菌中很少报道。在这项研究中，灰霉病菌（Botrytis cinerea）中一个推测的单羧酸转运基因[B。cinerea MctA (BcMctA)]是在一种B. cinerea transfer DNA （T-DNA）插入突变体的研究中发现的（74）。该基因的破坏降低了以单羧酸盐（醋酸盐或丙酮酸盐）为唯一碳源的培养基上的生长速度，但对乳酸盐没有影响。与野生菌株相比，BcmctA缺失突变体的丙酮酸含量降低了约35%。此外，BcmctA断裂突变体的分生孢子产量提高了约2倍。致病性试验结果表明，破坏BcmctA可显著降低黄瓜和番茄叶片上灰绿杆菌的毒力。我们的研究结果表明BcMctA与黄瓜和番茄叶片上葡萄球菌的丙酮酸吸收和致病性有关。
Monocarboxylate transporters have a central role in mammalian metabolism, but rarely reported in phytopathogenic fungi. In this study, a putative monocarboxylate transporter gene in Botrytis cinerea [B. cinerea MctA (BcMctA)] was identified in the research of a B. cinerea transfer DNA (T-DNA) insertional mutant (74). Disruption of the gene decreased the growth rate on the medium with monocarboxylate (acetate or pyruvate) as the sole carbon sources, but not affected on lactate. The pyruvate contents in BcmctA deletion mutants decreased about 35 % compared with the wild strain. Besides, the conidial yield was increased about two times in BcmctA disruption mutant. The pathogenicity assay indicated that disruption of BcmctA significantly reduced the virulence of B. cinerea on cucumber and tomato leaves. Our results demonstrated that BcMctA is related to pyruvate uptake and pathogenicity of B. cinerea on cucumber and tomato leaves.