Silane Layers on Silicon Surfaces: Mechanism of Interaction, Stability, and Influence on Protein Adsorption
Silane Layers on Silicon Surfaces: Mechanism of Interaction, Stability, and Influence on Protein Adsorption
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DOI：
10.1021/la2036778
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发表时间：
2012-01-10
期刊：
影响因子：
3.9
通讯作者：
Boujday, Souhir
中科院分区：
文献类型：
--
作者：
Aissaoui, Nesrine;Bergaoui, Latifa;Boujday, Souhir
本文从分子水平研究了3-氨丙基三乙氧基硅烷（APTES）与硅表面的相互作用机理。我们研究了实验参数，如时间，温度和浓度的APTES层的化学性质，形态和在水介质中的稳定性方面的质量的影响。这是使用最近开发的高灵敏度IR模式，掠射角衰减全反射（GA-ATR）实现的。该技术提供了关于所形成的APTES层的结构信息。该层的形貌进行了研究，在水介质中的原子力显微镜。用接触角测定法研究了其亲水性。结合这些技术，使硅烷接枝的机理的讨论。根据反应温度、室温或90 ℃，观察到相当大的差异。这些数据表明，存在两个吸附位点上的氧化硅层具有不同的亲和力。这也允许建立最佳参数以获得有序且稳定的硅烷层。APTES层上的蛋白质的吸附实现和监测使用原位石英晶体微天平与耗散监测和非原位GA-ATR分析。
In this work the mechanism of (3-aminopropyl)triethoxysilane (APTES) interaction with silicon surfaces is investigated at the molecular level. We studied the influence of experimental parameters such as time, temperature, and concentration on the quality of the APTES layer in terms of chemical properties, morphology, and stability in aqueous medium. This was achieved using a highly sensitive IR mode recently developed, grazing angle attenuated total reflection (GA-ATR). This technique provides structural information on the formed APTES layer. The topography of this layer was investigated by atomic force microscopy in aqueous medium. The hydrophilicity was also studied using contact angle measurement. Combining these techniques enables discussion of the mechanism of silane grafting. Considerable differences were observed depending on the reaction temperature, room temperature or 90 degrees C. The data suggest the presence of two adsorption sites with different affinities on the oxidized silicon layer. This also allows the optimal parameters to be established to obtain an ordered and stable silane layer. The adsorption of proteins on the APTES layer was achieved and monitored using in situ quartz crystal microbalance with dissipation monitoring and ex situ GA-ATR analyses.