Purification of the pro-phenol oxidase enzyme from haemocytes of the cockroach Blaberus discoidalis.
Purification of the pro-phenol oxidase enzyme from haemocytes of the cockroach Blaberus discoidalis.
复制标题
从蟑螂 Blaberus disoidalis 的血细胞中纯化前酚氧化酶。
DOI：
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发表时间：
1993
影响因子：
4.1
通讯作者：
Kenneth SODERHALLt
中科院分区：
文献类型：
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作者：
Hilary J. Durrant;N. Ratcliffe;C. Hipkin;Anna ASPANt;Kenneth SODERHALLt
Pro-phenol oxidase was purified from the haemocytes of the cockroach Blaberus discoidalis by Blue Sepharose chromatography, hydrophobic-interaction chromatography on a Phenyl-Superose column and, finally, gel filtration on a Superose 6 column. Results suggest that the molecule exists as a polymer of identical 76 kDa monomeric units. The enzyme is a glycoprotein with pI of 5.2 and can be converted by trypsin into phenol oxidase.
