Effect of Bone Morphogenetic Protein-12 Gene Transfer on Posterior Cruciate Ligament Healing in a Rabbit Model
Effect of Bone Morphogenetic Protein-12 Gene Transfer on Posterior Cruciate Ligament Healing in a Rabbit Model
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DOI：
10.1177/0363546508325960
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发表时间：
2009-03-01
影响因子：
4.8
通讯作者：
Ao, Yingfang
中科院分区：
文献类型：
--
作者：
Ma, Yong;Zhang, Xin;Ao, Yingfang
背景：后交叉韧带损伤后有一定程度的愈合。然而，保守治疗后的结果可能会随着长期随访而减少。骨形态发生蛋白-12可诱导韧带组织的形成，假设：骨形态发生蛋白-12基因转染可改善后交叉韧带愈合的组织学和生物力学性能，研究设计：对照实验研究。1条肢体的切断端接受含有3 × 10（7）pfu重组骨形态发生蛋白-12腺病毒的注射，对侧肢体的后交叉韧带作为未处理的对照。术后3、6、12、26周各处死8只家兔。另外，6只接受假手术的家兔用于获得正常对照数据。结果：26周时，治疗组修复组织在胶原排列、胶原形成、力学性能等方面与正常后交叉韧带组织相似。在第6、12和26周，治疗组的极限载荷、刚度和失效时吸收的能量显著大于未治疗组。腺病毒介导的骨形态发生蛋白-12基因转移在兔后交叉韧带部分撕裂模型中导致修复的韧带的组织学性质、拉伸强度和刚度的明显改善，临床相关性：骨形态发生蛋白-12基因转移是一种潜在的未来策略，以改善损伤后交叉韧带的修复。
Background: The posterior cruciate ligament heals to some extent after injury. However, results after conservative treatment may diminish with long-term follow-up. Bone morphogenetic protein-12 can induce formation of ligament tissues.Hypothesis: Bone morphogenetic protein-12 gene transfer can improve the histologic and biomechanical properties of healing posterior cruciate ligaments.Study Design: Controlled laboratory study.Methods: Bilateral posterior cruciate ligaments of 32 rabbits were injured. The cut ends in 1 limb received an injection containing 3 x 10(7) pfu recombinant bone morphogenetic protein-12 adenovirus, and the posterior cruciate ligament in the contralateral limb served as an untreated control. Eight rabbits were sacrificed at each time point of 3, 6, 12, and 26 weeks after the operation. In addition, 6 rabbits receiving a sham operation were used to obtain normal control data. The posterior cruciate ligament specimens were evaluated biomechanically and histologically.Results: The repair tissue of the treatment group at 26 weeks was similar to the normal posterior cruciate ligament in collagen arrangement, collagen formation, and mechanical properties. At weeks 6, 12, and 26, the ultimate load, stiffness, and energy absorbed at failure of the treatment group were significantly greater than those of the untreated group.Conclusion: Adenovirus-mediated bone morphogenetic protein-12 gene transfer in a partial posterior cruciate ligament laceration rabbit model resulted in an obvious improvement of histologic properties, tensile strength, and stiffness of the repaired ligaments, indicating improved posterior cruciate ligament healing.Clinical Relevance: Bone morphogenetic protein-12 gene transfer is a potential future strategy to improve the repair of injured posterior cruciate ligaments.