Dietary ω3 fatty acids modulate the substrate for post-operative atrial fibrillation in a canine cardiac surgery model
Dietary ω3 fatty acids modulate the substrate for post-operative atrial fibrillation in a canine cardiac surgery model
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DOI：
10.1093/cvr/cvq380
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发表时间：
2011-03-01
影响因子：
10.8
通讯作者：
Van Wagoner, David R.
中科院分区：
文献类型：
--
作者：
Mayyas, Fadia;Sakurai, Seiichiro;Van Wagoner, David R.
目的：据报道，饮食中的ω 3多不饱和脂肪酸（ω 3-PUFA）可降低心脏手术后新发房颤（AF）的发生率。在犬心脏手术模型中，我们评估了饮食ω 3-PUFA对心房电生理特性、炎症标志物、心房内皮素-1（ET-1）系统以及连接蛋白43的表达和分布的影响。（NC，n = 11）或补充有鱼油的食物（FO，0.6g omega 3 PUFA/kg/天，n = 9）。进行左胸廓切开术，切除左心耳（LAA）。放置心房起搏/记录导线，关闭心包/胸腔。Omega 6/Omega 3脂质的心房比率从NC的15-20降至FO的2-3。FO治疗降低了术前和稳定术后花生四烯酸水平。在FO处理的动物中，中性粒细胞与淋巴细胞的峰值比率较低，衰减更快。NC心房中存在广泛的炎性细胞浸润，但在FO处理的犬中减少。FO治疗的动物具有较低的术后心房诱导型一氧化氮合酶（iNOS）的表达和降低的血浆ET-1。ET-1和三磷酸肌醇受体2型蛋白在LAA中的表达也减少。FO治疗延长术后心房有效不应期，减慢心率，增强心率变异性。结论FO可通过调节心脏手术后的自主神经张力和心率，减轻心脏手术后诱发的AF。FO还减少心房炎症、iNOS和ET-1表达。
Aims Pre-treatment with dietary omega 3 polyunsaturated fatty acids (omega 3-PUFA) has been reported to reduce the incidence of new-onset atrial fibrillation (AF) following cardiac surgery. In a canine cardiac surgery model, we evaluated the impact of dietary omega 3-PUFA on atrial electrophysiological properties, inflammatory markers, the atrial endothelin-1 (ET-1) system, and the expression and distribution of connexin 43.Methods and results Adult mongrel dogs received either normal chow (NC, n = 11) or chow supplemented with fish oil (FO, 0.6 g omega 3PUFA/kg/day, n = 9) for 3 weeks before surgery. A left thoracotomy was performed, and the left atrial appendage (LAA) was excised. Atrial pacing/recording wires were placed, and the pericardium/chest was closed. The atrial ratio of omega 6/omega 3 lipids decreased from 15-20 in NC to 2-3 in FO. FO treatment lowered pre-surgical and stabilized post-surgical arachidonate levels. Peak neutrophil to lymphocyte ratio was lower and decayed faster in FO-treated animals. Extensive inflammatory cell infiltration was present in NC atria, but was reduced in FO-treated dogs. FO-treated animals had lower post-surgical atrial expression of inducible nitric oxide synthase (iNOS) and reduced plasma ET-1. Expression of ET-1 and inositol trisphosphate receptor type-2 proteins in the LAA was also reduced. FO treatment prolonged post-operative atrial effective refractory period, slowed heart rate, and enhanced heart rate variability. Importantly, AF (> 30 s) was inducible in four of six NC dogs, but no FO dogs.Conclusion Dietary FO attenuated AF inducibility following cardiac surgery by modulating autonomic tone and heart rate. FO also reduced atrial inflammation, iNOS, and ET-1 expression.