The effect of bovine parathyroid hormone withdrawal on MC3T3-E1 cell proliferation and phosphorus metabolism.
The effect of bovine parathyroid hormone withdrawal on MC3T3-E1 cell proliferation and phosphorus metabolism.
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牛甲状旁腺激素停药对MC3T3-E1细胞增殖和磷代谢的影响
DOI：
10.1371/journal.pone.0120402
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Shi W
中科院分区：
文献类型：
--
作者：
Liu S;Zhu W;Li S;Cui T;Li Z;Zhang B;Li Z;Wu J;Liang X;Lin Z;Shi W
低钙血症和低磷血症是甲状旁腺切除术（PTX）后常见的并发症。突然去除高循环水平的甲状旁腺激素（PTH）会导致骨吸收减少，导致骨重建空间减少。这些现象可能是由于钙和磷流入骨的增加。然而，目前没有数据支持这一假设。在这项研究中，我们发现PTX显著降低PTH、钙和磷酸盐的水平。与术前相比，术后1年PTH、钙和磷水平分别为295.6 ± 173.7 pg/mL（P < 0.05）、86.62 ± 15.98 mg/dL（P < 0.05）和5.56 ± 2.03 mg/dL（P < 0.05）。我们研究了连续牛甲状旁腺素管理以及撤回牛甲状旁腺素刺激小鼠成骨细胞前体细胞系MC 3 T3-E1。用连续牛PTH处理20天或用瞬时牛PTH处理10天培养MC 3 T3-E1细胞。高剂量的连续牛PTH暴露强烈降低细胞增殖，碱性磷酸酶活性和矿化钙结节的数量。然而，撤销牛PTH（100 ng/mL）显着增加矿化钙结节的数量，并导致培养基中钙和磷含量迅速下降。总之，连续暴露于牛PTH抑制成骨细胞分化，减少矿化结节的形成。然而，这种抑制被删除，矿化结节的形成恢复与撤回牛PTH。根据我们的临床检查和体外实验的结果，我们假设突然去除高水平的PTH可能会导致PTX后钙和磷流入骨的增加。
Hypocalcemia and hypophosphatemia are common complications after parathyroidectomy (PTX). Sudden removal of high circulating levels of parathyroid hormone (PTH) causes decreased osteoclastic resorption resulting in a decreased bone remodeling space. These phenomena are likely due to an increased influx of calcium and phosphorus into bone. However, there are currently no data to support this hypothesis. In this study, we found that PTX significantly reduced levels of PTH, calcium and phosphate. Compared with preoperative levels, after 1 year, postoperative PTH, calcium and phosphate levels were 295.6 ± 173.7 pg/mL (P < 0.05), 86.62 ± 15.98 mg/dL (P < 0.05) and 5.56 ± 2.03 mg/dL (P < 0.05), respectively. We investigated continuous bovine PTH administration as well as withdrawal of bovine PTH stimulation in the mouse osteoblast precursor cell line MC3T3-E1. MC3T3-E1 cells were cultured with continuous bovine PTH treatment for 20 days or with transient bovine PTH treatment for 10 days. High doses of continuous bovine PTH exposure strongly reduced cell proliferation, alkaline phosphatase activity and the number of mineralized calcium nodules. However, withdrawal of bovine PTH (100 ng/mL) significantly increased the number of mineralized calcium nodules and caused a rapid decline in calcium and phosphorus content of culture medium. In conclusion, continuous exposure to bovine PTH inhibited osteoblast differentiation and reduced the formation of mineralized nodules. However, this inhibition was removed and mineralized nodule formation resumed with withdrawal of bovine PTH. According to the results of our clinical examinations and in vitro experiments, we hypothesize that the sudden removal of high levels of PTH may cause an increased influx of calcium and phosphorus into bone after PTX.
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影响因子：
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