Age-dependent decrease in glomeruli and receptor cells containing α1-2fucose glycan in the mouse main olfactory system but not in the vomeronasal system
Age-dependent decrease in glomeruli and receptor cells containing α1-2fucose glycan in the mouse main olfactory system but not in the vomeronasal system
复制标题
小鼠主嗅觉系统中肾小球和含有 α1-2 岩藻糖聚糖的受体细胞的年龄依赖性减少，但犁鼻系统中没有
DOI：
10.1007/s00441-018-2819-9
复制
发表时间：
2018
影响因子：
3.6
通讯作者：
Nobuo Kitamura
中科院分区：
文献类型：
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作者：
Daisuke Kondoh;Motoki Sasaki;Nobuo Kitamura
嗅上皮(OE)和犁鼻器(VNO)的受体细胞分别向主嗅球(MOB)和副嗅球(AOB)的肾小球投射轴突，并在一生中经历不断的更替。α1-2岩藻糖(α1-2Fuc)多聚糖介导轴突生长和突触可塑性，在嗅觉系统发育过程中发挥重要作用。我们之前证实了α1-2Fuc多糖在3到4个月大的小鼠嗅觉系统中的定位，但这种定位是否会持续到一生仍是未知的。本文用能与α-1-2Fuc多糖特异结合的UEA-I对1、3和8月龄小鼠的MOB、AOB、OE和VNO进行了组织化学检查。各年龄组大鼠肾小球内UEA-I的结合部位相似，但UEA-I阳性肾小球的比例随着年龄的增长而显着降低。两个老年组OE中UEA-I阳性受体细胞的频率也显著低于1月龄小鼠。另一方面，AOB和VNO中的UEA-I结合在所有三组之间没有显著差异。这些发现表明，主要嗅觉系统的主要途径需要α1-2Fuc多糖在幼鼠身上发挥作用，而不是在老年鼠身上，而犁鼻途径在幼鼠和老年鼠中同样需要这种多糖。
Receptor cells of the olfactory epithelium (OE) and vomeronasal organ (VNO) project axons to glomeruli in the main olfactory bulb (MOB) and accessory olfactory bulb (AOB), respectively and undergo continuous turnover throughout life. Alpha1–2 fucose (α1–2Fuc) glycan mediates neurite outgrowth and synaptic plasticity and plays important roles in the formation of the olfactory system during development. We previously confirmed the localization of α1–2Fuc glycan in the olfactory system of 3- to 4-month-old mice but whether such localization persists throughout life remains unknown. Here, the MOB, AOB, OE and VNO of 1-, 3- and 8-month-old mice were histochemically examined usingUlex europaeusagglutinin-I (UEA-I) that specifically binds to α1–2Fuc glycan. Binding sites for UEA-I in the MOB were similar among all age groups but the ratio of UEA-I-positive glomeruli significantly decreased with aging. The frequency of UEA-I-positive receptor cells in the OE of the two older groups was also significantly lower than that of 1-month-old mice. On the other hand, UEA-I binding in the AOB and VNO did not significantly differ among all three groups. These findings suggest that the primary pathway of the main olfactory system requires the role of α1–2Fuc glycan in young mice rather than old mice, while the vomeronasal pathway equally requires this glycan in both young and old mice.