F-box proteins in rice. Genome-wide analysis, classification, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress
F-box proteins in rice. Genome-wide analysis, classification, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress
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DOI：
10.1104/pp.106.091900
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发表时间：
2007-04-01
期刊：
影响因子：
7.4
通讯作者：
Khurana, Jitendra P.
中科院分区：
文献类型：
--
作者：
Jain, Mukesh;Nijhawan, Aashima;Khurana, Jitendra P.
F-box蛋白在真核生物中构成一个大家族，其特征在于保守的F-box基序（约40个氨基酸）。作为Skp 1 p-cullin-F-box复合物的组成部分，F-box蛋白对于细胞蛋白质的受控降解至关重要。我们已经确定了687个潜在的F-box蛋白在水稻，模式单子叶植物，通过反复的数据库搜索。计算分析表明，F-box蛋白中还存在其它几个功能结构域，包括富亮氨酸重复序列、Kelch重复序列、F-box相关结构域、功能未知结构域和Tubby结构域。根据它们的结构域组成，它们被分为10个亚科。在F-box蛋白中发现了几个新的保守基序，这些基序不包含任何其他已知的功能结构域。对水稻F-box蛋白的分类、染色体定位、保守结构域和系统发育关系进行了分析。看来，F-box家族在水稻中的扩展，在很大程度上，可能是由于本地化的基因重复。此外，F-box蛋白编码基因的全面数字表达分析已经与微阵列分析互补。研究结果揭示了水稻F-box蛋白编码基因在花转变、穗和种子发育过程中的特异性和/或重叠表达。至少有43个F-box蛋白编码基因在不同的非生物胁迫条件下的水稻幼苗中差异表达。几个F-box蛋白编码基因的表达也受到光的影响。根据这些结果和已发表的资料，讨论了植物中F-box蛋白的结构和功能。这些数据将是有用的优先级的F-box蛋白在水稻中的功能验证。
F-box proteins constitute a large family in eukaryotes and are characterized by a conserved F-box motif (approximately 40 amino acids). As components of the Skp1p-cullin-F-box complex, F-box proteins are critical for the controlled degradation of cellular proteins. We have identified 687 potential F-box proteins in rice (Oryza sativa), the model monocotyledonous plant, by a reiterative database search. Computational analysis revealed the presence of several other functional domains, including leucine-rich repeats, kelch repeats, F-box associated domain, domain of unknown function, and tubby domain in F-box proteins. Based upon their domain composition, they have been classified into 10 subfamilies. Several putative novel conserved motifs have been identified in F-box proteins, which do not contain any other known functional domain. An analysis of a complete set of F-box proteins in rice is presented, including classification, chromosomal location, conserved motifs, and phylogenetic relationship. It appears that the expansion of F-box family in rice, in large part, might have occurred due to localized gene duplications. Furthermore, comprehensive digital expression analysis of F-box protein-encoding genes has been complemented with microarray analysis. The results reveal specific and/or overlapping expression of rice F-box proteinen-coding genes during floral transition as well as panicle and seed development. At least 43 F-box protein-encoding genes have been found to be differentially expressed in rice seedlings subjected to different abiotic stress conditions. The expression of several F-box protein-encoding genes is also influenced by light. The structure and function of F-box proteins in plants is discussed in light of these results and the published information. These data will be useful for prioritization of F-box proteins for functional validation in rice.