Enrichment of murine and human Langerhans cells with solid phase immunoabsorption using pan-leukocyte monoclonal antibodies.
Enrichment of murine and human Langerhans cells with solid phase immunoabsorption using pan-leukocyte monoclonal antibodies.
复制标题
使用泛白细胞单克隆抗体通过固相免疫吸收富集小鼠和人类朗格汉斯细胞。
DOI：
10.1111/1523-1747.ep12274673
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Morhenn,VB
中科院分区：
文献类型：
--
作者：
Wood,GS;Kosek,J;Butcher,EC;Morhenn,VB
利用固相免疫吸收（panning）技术，我们利用泛白细胞单克隆抗体从正常皮肤细胞悬浮液中富集和消耗小鼠和人朗格汉斯细胞。朗格汉斯细胞富集组分含有80-99%的单核细胞，几乎全部具有朗格汉斯细胞的超结构特征。这些结果与用抗leu -6（T6）抗体筛选人朗格汉斯细胞所获得的结果相当。同样，在郎格汉斯细胞缺失的部分中检测到不到1%的这些细胞，这些细胞在皮肤细胞-淋巴细胞反应中不能刺激异体淋巴细胞。我们得出结论，泛白细胞抗体淘洗是从异质皮肤细胞悬浮液中富集或消耗朗格汉斯细胞的有效手段，并且可以产生与使用更多朗格汉斯细胞特异性试剂（如抗leu -6(T6)）相似的结果。这些发现对小鼠朗格汉斯细胞的富集和缺失具有特别重要的意义，因为它们不表达与人类Leu-6（T6）抗原的已知相关性。
Using a solid phase immunoabsorption (panning) technique, we have employed pan-leukocyte monoclonal antibodies to enrich and deplete murine and human Langerhans cells from cell suspensions of normal skin. Langerhans cell-enriched fractions contained 80–99% mononuclear cells, almost all of which had the ultra- structural features of Langerhans cells. These results are comparable to those achieved by panning for human Langerhans cells with anti-Leu-6(T6) antibody. Similarly, less than 1% of these cells were detectable in Langerhans cell-depleted fractions and such fractions were incapable of stimulating allogeneic lymphocytes in the skin cell-lymphocyte reaction. We conclude that panning with pan-leukocyte antibodies is an effective means of enriching or depleting Langerhans cells from heterogeneous skin cell suspensions and can yield results similar to those achieved with more Langerhans cell-specific reagents such as anti-Leu-6(T6). These findings are of particular significance to the enrichment and depletion of murine Langerhans cells since they express no known correlate of the human Leu-6(T6) antigen.