Arabidopsis Paired Amphipathic Helix Proteins SNL1 and SNL2 Redundantly Regulate Primary Seed Dormancy via Abscisic Acid-Ethylene Antagonism Mediated by Histone Deacetylation
Arabidopsis Paired Amphipathic Helix Proteins SNL1 and SNL2 Redundantly Regulate Primary Seed Dormancy via Abscisic Acid-Ethylene Antagonism Mediated by Histone Deacetylation
复制标题
拟南芥配对两性螺旋蛋白 SNL1 和 SNL2 通过组蛋白脱乙酰化介导的脱落酸-乙烯拮抗作用冗余调节初级种子休眠
DOI：
10.1105/tpc.112.108191
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发表时间：
2013-01-01
期刊：
影响因子：
11.6
通讯作者：
Liu, Yong-Xiu
中科院分区：
文献类型：
--
作者：
Wang, Zhi;Cao, Hong;Liu, Yong-Xiu
组蛋白乙酰化（去乙酰化）是一种高度保守的染色质修饰，对发育和生长至关重要。在这项研究中，我们确定了两个成员的组蛋白去乙酰化复合物在拟南芥，SIN 3-LIKE 1（SNL 1）和SNL 2种子休眠的作用。与野生型相比，双突变体snl 1 snl 2显示出降低的休眠和对组蛋白脱乙酰基酶抑制剂阿司他丁A和二烯丙基二硫化物的超敏反应。SNL 1在体外和植物中与组蛋白脱乙酰酶19相互作用，SNL 1和SNL 2的功能丧失突变体显示组蛋白3赖氨酸9/18（H3 K9/18）和H3 K14的乙酰化水平增加。此外，SNL 1和SNL 2通过降低其组蛋白乙酰化水平来调节参与乙烯和脱落酸（阿坝）途径的关键基因。综上所述，我们发现SNL 1和SNL 2通过介导ABA-乙烯拮抗作用调节拟南芥种子休眠。SNL 1和SNL 2可能代表阿坝和乙烯途径在种子休眠调控中的一个交叉点。
Histone (de)acetylation is a highly conserved chromatin modification that is vital for development and growth. In this study, we identified a role in seed dormancy for two members of the histone deacetylation complex in Arabidopsis thaliana, SIN3-LIKE1 (SNL1) and SNL2. The double mutant snl1 snl2 shows reduced dormancy and hypersensitivity to the histone deacetylase inhibitors trichostatin A and diallyl disulfide compared with the wild type. SNL1 interacts with HISTONE DEACETYLASE19 in vitro and in planta, and loss-of-function mutants of SNL1 and SNL2 show increased acetylation levels of histone 3 lysine 9/18 (H3K9/18) and H3K14. Moreover, SNL1 and SNL2 regulate key genes involved in the ethylene and abscisic acid (ABA) pathways by decreasing their histone acetylation levels. Taken together, we showed that SNL1 and SNL2 regulate seed dormancy by mediating the ABA-ethylene antagonism in Arabidopsis. SNL1 and SNL2 could represent a cross-link point of the ABA and ethylene pathways in the regulation of seed dormancy.