LRG1 suppresses the migration and invasion of hepatocellular carcinoma cells
LRG1 suppresses the migration and invasion of hepatocellular carcinoma cells
复制标题
LRG1抑制肝癌细胞的迁移和侵袭
DOI：
10.1007/s12032-015-0598-7
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发表时间：
2015-05-01
期刊：
影响因子：
3.4
通讯作者：
Qin, Wenxin
中科院分区：
文献类型：
--
作者：
Zhang, Yurong;Luo, Qin;Qin, Wenxin
肝细胞癌（Hepatocellular carcinoma，HCC）是一种由复杂的病理机制驱动的恶性肿瘤，具有进展快、预后差的特点。肝癌患者死亡的主要原因是肿瘤转移。然而，HCC转移的潜在分子机制在很大程度上是未知的。在本研究中，一个新的转移相关基因，富含亮氨酸的α-2-糖蛋白1（LRG 1），被确定在肝癌。我们通过实时定量PCR和免疫组化染色发现LRG 1在肝癌组织中表达下调。体外实验表明，LRG 1对细胞增殖没有影响。LRG 1的异位过表达降低了肝癌细胞的迁移和侵袭能力，而沉默LRG 1可增强肝癌细胞的迁移和侵袭能力。外源性重组人LRG 1蛋白在体外可抑制肝癌细胞的迁移和侵袭。上述结果表明LGR 1参与了肝癌转移的抑制，可能是一种新的肝癌转移抑制因子。
Hepatocellular carcinoma (HCC) is a malignant tumor driven by complex pathological mechanisms and is characterized by fast progression and poor prognosis. The main cause of death in HCC patients is tumor metastasis. However, underlying molecular mechanisms of metastasis are largely unknown in HCC. In the present study, a novel metastasis-related gene, leucine-rich-alpha-2-glycoprotein 1 (LRG1), was identified in HCC. We revealed that LRG1 expression was downregulated in HCC tissues by quantitative real-time PCR and immunohistochemical staining. In vitro assays demonstrated LRG1 had no effect on cell proliferation. Migratory and invasive potential of HCC cells was reduced by ectopic overexpression of LRG1, whereas silencing LRG1 could enhance migration and invasion of HCC cells. Furthermore, exogenous recombinant human protein of LRG1 could inhibit migration and invasion of HCC cells in vitro. The above findings indicate that LGR1 is involved in the inhibition of HCC metastasis and it may function as a novel metastasis suppressor in HCC.