Screen-printed enzyme electrodes for the detection of marker analytes during winemaking
Screen-printed enzyme electrodes for the detection of marker analytes during winemaking
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DOI：
10.1016/j.aca.2003.09.030
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发表时间：
2004-06-18
影响因子：
6.2
通讯作者：
Palleschi, G
中科院分区：
文献类型：
--
作者：
Lupu, A;Compagnone, D;Palleschi, G
利用丝网印刷电极和两种不同类型的酶：依赖NAD(P)(+)的脱氢酶和氧化酶，已经开发出苹果酸和葡萄糖的生物传感器。用麦尔多拉蓝(苹果酸)和普鲁士蓝(葡萄糖)修饰电极的活性表面，得到了灵敏、低成本、可靠的NAD(P)H和H_2O_2探针。固定电位安培法用于检测小体积样品(50微升)中的底物。将这些酶固定在聚乙烯亚胺-戊二醛交联膜中，可以获得具有足够操作稳定性的传感器。苹果酸和葡萄糖的检出限分别为10(-5)M和10(-6)M。该传感器被应用于不同葡萄酒样品的分析。(C)2003爱思唯尔B.V.保留所有权利。
Biosensors for malic acid and glucose have been developed, using screen-printed electrodes and two different classes of enzymes: NAD(P)(+)-dependent dehydrogenases and oxidases. The active surface of the electrodes was modified using Meldola Blue (malic acid) and Prussian Blue (glucose) and in this way sensitive, low cost and reliable NAD(P)H and H2O2 probes were obtained. Fixed potential amperometry was used for the detection of substrates in small volumes of sample (50 mul). Immobilization of the enzymes in a polyethylenimine-glutaraldehyde cross-linking membrane allowed sensors to be obtained with sufficient operational stability. The detection limits were of 10(-5) M for malic acid and 10(-6) M for glucose. The sensors were applied in the analysis of different samples of wine. (C) 2003 Elsevier B.V. All rights reserved.