Inhibition of VP and OT release by water in hypovolemia is independent of opioid peptides.
Inhibition of VP and OT release by water in hypovolemia is independent of opioid peptides.
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血容量不足时水对 VP 和 OT 释放的抑制与阿片肽无关。
DOI：
10.1152/ajpregu.1987.253.1.r31
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Summy-Long,JY
中科院分区：
文献类型：
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作者：
Rosella-Dampman,LM;Hartman,RD;Summy-Long,JY
在血容量不足时，过度水化抑制了下丘脑-神经垂体系统血管加压素（VP）和催产素（OT）的释放。我们研究了阿片肽是否介导水对这些激素分泌的抑制作用。通过出血（HEM，0.51 ml/min）20%和35%的血容量或通过皮下注射聚乙二醇（PEG，20，000 mol wt，35 ml/kg）或腹膜内注射组胺（HIS，15 mg/kg，1 ml/kg）使清醒的雄性大鼠低血容量。动物经口插管1-4分钟（HEM，HIS）或6.75小时（PEG）后，有或没有给予水（40 ml/kg）。气管插管后4 ~ 7 min分别注射生理盐水（1 ml/kg）或纳洛酮（2或5 mg/kg），6-10 min后断头处死。对照组动物进行类似处理，但未受到血容量减少刺激。从血浆中提取VP和OT，并通过放射免疫法进行定量。数据采用方差分析。在HEM动物中，血压下降，血浆渗透压升高，两者均与血浆[VP]和[OT]的升高呈正相关。过度水化降低血浆渗透压，减弱血压下降，并减少HEM动物血浆中的[VP]和[OT]。阿片受体拮抗剂，纳洛酮，并没有改变这些变化的血压或血浆渗透压，或血浆[VP]后HEM在大鼠治疗或不治疗水。然而，在正常水合和过度水合大鼠中，纳洛酮均增加血浆[OT]。因此，无论动物的水合状态，阿片肽抑制释放OT，但不VP出血期间。还从用PEG或HIS造成低血容量的大鼠中获得了与该解释一致的数据。（250字处删节）
Overhydration inhibits release of vasopressin (VP) and oxytocin (OT) from the hypothalamo-neurohypophysial system during hypovolemia. We investigated whether opioid peptides mediate the inhibitory effect of water on secretion of these hormones. Conscious male rats were made hypovolemic by hemorrhage (HEM, 0.51 ml/min) of 20 and 35% of the blood volume or by injection of either subcutaneous polyethylene glycol (PEG, 20,000 mol wt, 35 ml/kg) or intraperitoneal histamine (HIS, 15 mg/kg, 1 ml/kg). Animals were intubated orally 1-4 min (HEM, HIS) or 6.75 h (PEG) later with or without administration of water (40 ml/kg). Four to seven min after intubation rats were injected with saline (1 ml/kg) or naloxone (2 or 5 mg/kg) and then decapitated 6-10 min later. Control animals were treated similarly but were not stimulated by hypovolemia. VP and OT were extracted from plasma and quantified by radioimmunoassay. Data were analyzed by analysis of variance. In HEM animals blood pressure fell and plasma osmolality increased, both of which correlated positively with the rise in plasma [VP] and [OT]. Overhydration lowered the plasma osmolality, attenuated the fall in blood pressure, and reduced [VP] and [OT] in plasma of HEM animals. The opiate receptor antagonist, naloxone, did not alter these changes in blood pressure or plasma osmolality, or the plasma [VP] after HEM in rats treated with or without water. Plasma [OT] was, however, increased by naloxone in both normally hydrated and overhydrated rats. Thus, regardless of the hydrational state of the animal, opioid peptides inhibited release of OT but not VP during hemorrhage. Data consistent with this interpretation were also obtained from rats made hypovolemic with PEG or HIS.(ABSTRACT TRUNCATED AT 250 WORDS)