A PROTEIN-A-BINDING, POLYETHYLENE-GLYCOL PRECIPITATION-BASED IMMUNORADIOMETRIC ASSAY - APPLICATION TO THE DETECTION OF IMMUNE-COMPLEXES AND C-3 IN HUMAN-SERA AND OF PRIVATE ANTIGENS IN CROSS-REACTING PARASITE EXTRACTS
A PROTEIN-A-BINDING, POLYETHYLENE-GLYCOL PRECIPITATION-BASED IMMUNORADIOMETRIC ASSAY - APPLICATION TO THE DETECTION OF IMMUNE-COMPLEXES AND C-3 IN HUMAN-SERA AND OF PRIVATE ANTIGENS IN CROSS-REACTING PARASITE EXTRACTS
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DOI：
10.1016/0022-1759(86)90028-1
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发表时间：
1986-01-01
影响因子：
2.2
通讯作者：
GALVAOCASTRO, B
中科院分区：
文献类型：
--
作者：
PONTESDECARVALHO, LC;LANNESVIEIRA, J;GALVAOCASTRO, B
一种免疫放射分析，基于沉淀的抗原-抗体复合物的聚乙二醇（PEG）和随后结合的PEG-可溶性放射性标记的葡萄球菌蛋白A的PEG-不溶性复合物，描述。该测定可应用于检测天然存在的循环免疫复合物，以及通过混合抗原和抗体溶液人工产生的复合物，这使得其在几种情况下可用于检测抗原或抗体。用3%PEG预处理含抗体的血清，大大降低了背景值，提高了测定的灵敏度。该方法也可用于检测和分离（1）与相关寄生虫克氏锥虫抗原无交叉反应的杜氏利什曼原虫抗原（特异性抗原）和（2）昆虫源性亚环锥鞭毛体的特异性抗原。cruzi与T.克鲁兹一个简单而非常有效的程序，洗涤沉淀物，只有一个离心也被描述。
An immunoradiometric assay, based on the precipitation of antigen-antibody complexes by polyethylene glycol (PEG) and on the subsequent binding of PEG-soluble radiolabelled staphylococcal protein A to the PEG-insoluble complexes, is described. The assay can be applied to the detection of naturally occurring, circulating immune complexes, and of complexes artificially created by mixing antigen and antibody solutions, which makes it of potential use for the detection of either antigen or antibody in several situations. Pre-treatment of the antibody-containing sera with 3% PEG greatly reduced the background values and increased the sensitivity of the assay. The assay was also applied to the detection and isolation of (1) Leishmania donovani antigens that did not cross-react with antigens of the related parasite Trypanosoma cruzi (private antigens) and (2) private antigens of insect-derived metacyclic trypomastigotes of T. cruzi in relation to culture-derived metacyclic trypomastigotes of T. cruzi. A simple and extremely effective procedure for washing precipitates with just one centrifugation is also described.