Pattern of somatic transposition in a high copy Ac tomato line.
Pattern of somatic transposition in a high copy Ac tomato line.
复制标题
高拷贝 Ac 番茄品系中体细胞转座的模式。
DOI：
10.1046/j.1365-313x.1992.t01-40-00999.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
John I. Yoder
中科院分区：
文献类型：
--
作者：
François Belzile;John I. Yoder
先前已经描述了每个基因组含有8至10个拷贝的异常活性的玉米转座因子Ac的转基因番茄系。Southern分析表明，这些元素在这些植物中具有体细胞活性。为了进一步表征该品系中体细胞转座的模式，克隆了来自单个植物的24个独立的Ac插入事件。在21例中，Ac插入单拷贝基因组DNA，而在3例中Ac插入每个基因组2至4个拷贝的序列中;没有一个插入发生在更高度重复的DNA中。20个插入位点的染色体位置确定的RFLP作图和小分散的集群出现的模式。20个插入位点中有13个与至少一个其他插入位点连锁，但这些插入位点分布在12条番茄染色体中的9条上。只有一个Ac插入连接到T-DNA位点。这些Ac元件的结构完整性进行了检查，并没有证据的删除或其他重排暗示Ds elements. The的影响，这些研究结果与使用Ac作为转座子标签在异源物种进行了讨论。
A transgenic tomato line containing between eight and ten copies per genome of an exceptionally active maize transposable element Ac has previously been described. Southern analyses indicated that these elements are somatically active in these plants. In order to characterize further the pattern of somatic transposition in this line, 24 independent Ac insertion events from a single plant were cloned. In 21 cases, Ac inserted into single copy genomic DNA while in three cases Ac inserted into sequences present at two to four copies per genome; none of the insertions occurred into more highly repetitive DNA. The chromosomal locations of 20 insertion sites were determined by RFLP mapping and a pattern of small dispersed clusters emerged. Thirteen of the 20 insertion sites were linked to at least one other insertion site but these were distributed over nine of the 12 tomato chromosomes. Only one Ac insertion was linked to the T-DNA locus. The structural integrity of these Ac elements was examined and no evidence of deletions or other rearrangements suggestive of Ds elements was found. The implications of these findings with respect to the use of Ac as a transposon tag in heterologous species are discussed.