MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene
MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene
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DOI：
10.3109/08977194.2013.860453
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发表时间：
2014-02-01
期刊：
影响因子：
1.8
通讯作者：
Meng, Xingyu
中科院分区：
文献类型：
--
作者：
Hu, Wei;Li, Ting;Meng, Xingyu
MicroRNA及其在鹿茸快速生长和再生中的作用引起了人们的广泛关注。在本研究中，我们研究了microRNAs let-7a和let-7 f对鹿茸细胞增殖的影响。我们使用荧光素酶报告筛选来证明胰岛素样生长因子1受体（IGF-1 R）可以由let-7a和let-7 f调节。MTT法证实let-7a和let-7 f模拟物可抑制软骨细胞增殖。相比之下，let-7a和let-7 f抑制剂的转染增加了软骨细胞的增殖，表明抑制剂可以竞争性结合内源性miRNA，从而降低miRNA的抑制作用。此外，蛋白质印迹分析进一步鉴定了let-7a和let-7 f模拟物抑制IGF-1 R表达，并且let-7a和let-7 f抑制剂增加IGF-1 R的表达水平。本研究表明let-7a和let-7 f在鹿茸增殖过程中起重要作用，在鹿茸发育中具有潜在的应用价值。let-7a和let-7 f可能是鹿茸IGF-1 R表达的新调控因子。
MicroRNAs and their roles in rapid antler growth and regeneration have attracted much attention. In the present study, we examined the effects of microRNAs let-7a and let-7f on antler cell proliferation. We used a luciferase reporter screen to demonstrate that insulin-like growth factor 1 receptor (IGF-1R) can be regulated by let-7a and let-7f. MTT assay confirmed that chondrocyte proliferation was inhibited by let-7a and let-7f mimics. In contrast, transfection of let-7a and let-7f inhibitors increased chondrocyte proliferation, indicating that inhibitors can competitively bind to endogenous miRNA, reducing the inhibitory effect of miRNA. Moreover, western blotting analysis further identified that let-7a and let-7f mimics suppressed IGF-1R expression, and that let-7a and let-7f inhibitors increased the expression level of IGF-1R. Taken together, our study demonstrates the important roles of let-7a and let-7f in antler proliferation and its potential application in antler development. let-7a and let-7f may represent novel regulatory factors of IGF-1R expression in deer antler.