Clostridium botulinum type D toxin: purification, molecular structure, and some immunological properties
Clostridium botulinum type D toxin: purification, molecular structure, and some immunological properties
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D 型肉毒梭菌毒素：纯化、分子结构和一些免疫学特性
DOI：
10.1128/iai.17.2.395-401.1977
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发表时间：
1977
影响因子：
3.1
通讯作者：
G. Sakaguchi
中科院分区：
文献类型：
--
作者：
S. Miyazaki;M. Iwasaki;G. Sakaguchi
肉毒杆菌D型祖毒素的纯化。在整个培养物中加入核糖核酸有助于毒素的初始酸沉淀。与B型一样，L （16S）和M毒素（12S）均来自血凝素阳性菌株，而M毒素仅由血凝素阴性菌株产生。M毒素（分子量，30万）由有毒成分（分子量，17万）和无毒成分（分子量，13万）各一个分子组成；L毒素由两种成分和血凝素组成。M毒素的特异毒性为5 × 10(8)平均致死剂量/ mg N；L毒素的平均致死剂量为每mg n 2.4 X 10(8)，这些毒素完全或几乎完全有效，但以非缺口形式存在。胰蛋白酶化导致有毒成分出现缺口，形成由两条肽链组成的分子，分子量分别为110,000和60,000；毒性几乎没有增加。D型毒株的毒性成分与C型毒株的毒性成分在抗原性上无相关性，而无毒成分与C型毒株的毒性成分在抗原性上无明显区别。两种毒素均不含C和D菌株阐述的C2毒性因子。
Clostridium botulinum type D progenitor toxin was purified. The addition of ribonucleic acid to the whole culture helped initial acid precipitation of the toxin. As with type B, both L (16S) and M toxins (12S) obtained from a hemagglutinin-positive strain, whereas M toxin only was produced by a hemagglutinin-negative strain. M toxin (molecular weight, 300,000) consisted of one molecule each of a toxic (molecular weight, 170,000) and a nontoxic component (molecular weight, 130,000); L toxin consisted of both components plus hemagglutinin. The specific toxicity of M toxin was 5 X 10(8) mean lethal doses per mg of N; that of L toxin was 2.4 X 10(8) mean lethal doses per mg of N. These toxins were fully or nearly fully active, but in un-nicked form. Trypsinization caused nicking in the toxic component, forming a molecule made up of two peptide chains with molecular weights of 110,000 and 60,000; there was little or no increase in toxicity. The toxic component of type D was not antigenically related to that of type C, whereas the nontoxic component was antigenically indistinguishable from that of type C. The toxicities of both L nad M toxins of the hemagglutinin-positive strain were increased twofold by trypsinization. Neither toxin contained the C2 toxic factor elaborated by C and D strain.